Long noncoding RNA interplay with the host microbiome may determine mucosal influenza vaccine immunogenicity

• Grant number: 17/50137-3
• Support type: Regular Research Grants
• Duration: September 01, 2017 - August 31, 2019
• Field of knowledge: Biological Sciences - Immunology
• Cooperation agreement: Imperial College, UK
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Helder Takashi Imoto Nakaya
• Grantee: Helder Takashi Imoto Nakaya
• Principal investigator abroad: Thushan De Silva
• Institution abroad: Imperial College London, England
• Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 12/19278-6 - Systems biology of long non-coding RNAs, AP.JP

Abstract

This project will foster an independently funded collaboration between laboratories of Dr. Thushan de Silva and Dr. Beate Kampmann at Imperial College London and Dr. Helder Nakaya's group (FCF-USP) at USP. By applying a cutting edge systems biology approach to integrate nasopharyngeal microbiome, mucosal and blood transcriptome, immunological and clinical data, we will identify and investigate the putative role of long noncoding RNAs (IncRNAs) in vaccine-induced mucosal immunity. The exchange visits for data analysis/discussion sections, as well as the lectures/seminars and the workshop offered will provide the framework to spark the interaction between the groups. We will combine the expertise from Nakaya laboratory at USP and the data being generated via a clinical trial of LAIV (Live attenuted influenza vaccine) in children aged 24 - 59 months conducted at the MRC Unit the Gambia. Also, close links established during this proposal will enable Brazilian research to benefit from the well- established global health research network maintained by professor Kampmann, which includes the Centre for International Child Health (CICH) at Imperial College London recently established under her directorship. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LIMA, DIOGENES S.; CARDOZO, LUCAS E.; MARACAJA-COUTINHO, VINICIUS; SUHRBIER, ANDREAS; MANE, KARIM; JEFFRIES, DAVID; SILVEIRA, EDUARDO L. V.; AMARAL, PAULO P.; RAPPUOLI, RINO; DE SILVA, THUSHAN I.; NAKAYA, HELDER I. Long noncoding RNAs are involved in multiple immunological pathways in response to vaccination. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 116, n. 34, p. 17121-17126, AUG 20 2019. Web of Science Citations: 0.

MASTELIC-GAVILLET, BEATRIS; VONO, MARIA; GONZALEZ-DIAS, PATRICIA; FERREIRA, FREDERICO MORAES; CARDOZO, LUCAS; LAMBERT, PAUL-HENRI; NAKAYA, I, HELDER; SIEGRIST, CLAIRE-ANNE. Neonatal T Follicular Helper Cells Are Lodged in a Pre-T Follicular Helper Stage Favoring Innate Over Adaptive Germinal Center Responses. FRONTIERS IN IMMUNOLOGY, v. 10, AUG 13 2019. Web of Science Citations: 0.

SOARES-SCHANOSKI, ALESSANDRA; BAPTISTA CRUZ, NATALIA; DE CASTRO-JORGE, LUIZA ANTUNES; HOMEM DE CARVALHO, RENAN VILLANOVA; DOS SANTOS, CLIOMAR ALVES; DA ROS, NANCY; OLIVEIRA, URSULA; COSTA, DANUZA DUARTE; SIMOES DOS SANTOS, CECILIA LUIZA; CUNHA, MARIELTON DOS PASSOS; SARNO OLIVEIRA, MARIA LEONOR; ALVES, JULIANA CARDOSO; DE LUCENA COUTO OCEA, REGINA ADALVA; RIBEIRO, DANIELLE RODRIGUES; AQUIME GONCALVES, ANDRE NICOLAU; GONZALEZ-DIAS, PATRICIA; SUHRBIER, ANDREAS; DE ANDRADE ZANOTTO, PAOLO MARINHO; DE AZEVEDO, INACIO JUNQUEIRA; ZAMBONI, DARIO S.; ALMEIDA, ROQUE PACHECO; HO, PAULO LEE; KALIL, JORGE; NISHIYAMA, MILTON YUTAKA; NAKAYA, HELDER I. Systems analysis of subjects acutely infected with the Chikungunya virus. PLOS PATHOGENS, v. 15, n. 6 JUN 2019. Web of Science Citations: 1.

FUKUTANI, KIYOSHI F.; NASCIMENTO-CARVALHO, CRISTIANA M.; BOUZAS, MAIARA L.; OLIVEIRA, JULIANA R.; BARRAL, ALDINA; DIERCKX, TIM; KHOURI, RICARDO; NAKAYA, HELDER I.; ANDRADE, BRUNO B.; VAN WEYENBERGH, JOHAN; DE OLIVEIRA, CAMILA I. In situ Immune Signatures and Microbial Load at the Nasopharyngeal Interface in Children With Acute Respiratory Infection. FRONTIERS IN MICROBIOLOGY, v. 9, NOV 9 2018. Web of Science Citations: 0.