| Grant number: | 12/19278-6 |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| Duration: | December 01, 2013 - November 30, 2018 |
| Field of knowledge: | Interdisciplinary Subjects |
| Principal Investigator: | Helder Takashi Imoto Nakaya |
| Grantee: | Helder Takashi Imoto Nakaya |
| Host Institution: | Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated researchers: | Marina Baquerizo Martinez ; Mario Hiroyuki Hirata ; Vinicius Ramos Henriques Maracajá Coutinho |
| Associated grant(s): | 18/14933-2 - Integrative biology applied to human health,
AP.JP2 17/50137-3 - Long noncoding RNA interplay with the host microbiome may determine mucosal influenza vaccine immunogenicity, AP.R SPRINT 14/50308-4 - Transcriptomic meta-analysis of positionally conserved long noncoding RNAs, AP.R |
| Associated scholarship(s): | 14/19323-7 - Long noncoding RNAs as potential biomarkers of arthritis, BP.IC |
Abstract
It is estimated that thousands of long non-coding RNAs (lncRNAs) are transcribed in the genome of several organisms. Yet, only a small fraction of them have been functionally characterized. One of the main reasons for this lies in our poor understanding of their expression patterns under different biological conditions, and the difficulty in identifying their possible gene targets. Most of the high-throughput techniques, such as microarrays and next-generation sequencing (RNA-seq), aim to monitor the expression of protein-coding genes. Some of this data, although unexplored in the original publications, are derived from lncRNAs. In this project, we propose to utilize publicly-available high-throughput data to study the systems biology of lncRNAs under different perturbations and the implication of lncRNAs in the regulation of candidate target genes. For example, we re-annotated the most used Affymetrix chip, with which more than 2,300 studies have been published, and revealed the existence of 10,248 probe sets of putative lncRNAs. Studying the expression profile of lncRNAs and how their expression correlates with the expression of protein-coding genes in various tissues and conditions will reveal interesting mechanisms of gene regulation. These analyses will involve the identification of co-expression modules, the prediction of transcription factors responsible for lncRNA transcription, and the association of lncRNAs to diseases and infections. We also propose to validate the regulatory role of some of these lncRNAs. (AU)
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