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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance

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Author(s):
Reis Borges, Karina Lima [1] ; Salvato, Fernanda [2, 1] ; Loziuk, Philip L. [3] ; Muddiman, David C. [3] ; Azevedo, Ricardo Antunes [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083862 Campinas, SP - Brazil
[3] North Carolina State Univ, Dept Chem, WM Keck FTMS Lab Human Hlth Res, Raleigh, NC 27695 - USA
Total Affiliations: 3
Document type: Journal article
Source: Environmental Science and Pollution Research; v. 26, n. 25, p. 26039-26051, SEP 2019.
Web of Science Citations: 0
Abstract

This is a report on comprehensive characterization of cadmium (Cd)-exposed root proteomes in tomato using label-free quantitative proteomic approach. Two genotypes differing in Cd tolerance, Pusa Ruby (Cd-tolerant) and Calabash Rouge (Cd-sensitive), were exposed during 4 days to assess the Cd-induced effects on root proteome. The overall changes in both genotypes in terms of differentially accumulated proteins (DAPs) were mainly associated to cell wall, redox, and stress responses. The proteome of the sensitive genotype was more responsive to Cd excess, once it presented higher number of DAPs. Contrasting protein accumulation in cellular component was observed: Cd-sensitive enhanced intracellular components, while the Cd-tolerant increased proteins of extracellular and envelope regions. Protective and regulatory mechanisms were different between genotypes, once the tolerant showed alterations of various protein groups that lead to a more efficient system to cope with Cd challenge. These findings could shed some light on the molecular basis underlying the Cd stress response in tomato, providing fundamental insights for the development of Cd-safe cultivars. (AU)

FAPESP's process: 09/54676-0 - Oxidative stress induced by metals: new approaches
Grantee:Ricardo Antunes de Azevedo
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/14349-3 - Cadmium transport and accumulation of different tomato genotypes: physiological, proteomic and molecular approaches.
Grantee:Ricardo Antunes de Azevedo
Support Opportunities: Regular Research Grants