Proteomic, metabolic and immunological changes in Biomphalaria glabrata infected with Schistosoma mansoni

Author(s)

Fernandes Mendes, Tiago Manuel ^[1] ; Carrilho, Emanuel ^{[2, 3]} ; Fonseca Sieuve Afonso, Ana Julia Pinto ^{[2, 4]} ; Galinaro, Carlos Alexandre ^{[2, 3]} ; Cabral, Fernanda Janku ^[1] ; Allegretti, Silmara Marques ^[1]

Affiliation

^[1] Campinas State Univ UNICAMP, Biol Inst, Anim Biol Dept, Campinas, SP - Brazil

^[2] Univ Sao Paulo, Sao Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[3] Inst Ciencia & Tecnol Bioanalit INCTBio, BR-13083590 Campinas, SP - Brazil

^[4] Univ Nova Lisboa, IHMT, GHTM, Med Microbiol Unit, Lisbon - Portugal

• Document type: Journal article
• Source: International Journal for Parasitology; v. 49, n. 13-14, p. 1049-1060, DEC 2019.
• Web of Science Citations: 0

Abstract

Mansonic schistosomiasis is a neglected disease transmitted by Biomphalaria spp. snails. Understanding what happens inside the intermediate host is important to develop more efficient ways of reducing schistosomiasis prevalence. Our purpose was to characterize metabolic and immunological changes in Biomphalaria glabrata 24 h after exposure to Schistosoma mansoni. For this purpose, proteins were extracted from snails' whole tissue with Tris-Urea buffer and digested with tripsin. Mass spectrometry was performed and analyzed with MaxQuant and Perseus software. Also, the hemolymph of five snails 24 h post exposure was collected, and the numbers of hemocytes, levels of urea, uric acid, nitric oxide, calcium, glycogen and alanine and aspartate aminotransferases activities were assessed. Snails were also dissected for measurement of glycogen content in the cephalopodal region and gonoda-digestive gland complex. Globin domain proteins were found to be up-regulated: also the number of circulating hemocytes was significantly higher after 24 h of exposure to the parasite. NO levels were higher 24 h post exposure. Several proteins associated with energy metabolism were found to be up-regulated. Glycogen analysis showed a significant decrease in the gonad-digestive gland complex glycogen content. We found several proteins which seem to be associated with the host immune response, most of which were up-regulated, however some were down-regulated, which may represent an important clue in understanding B. glabrata - S. mansoni compatibility. (C) 2019 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/54040-8 - Acquisition of a high-resolution Orbitrap mass spectrometer for the discovery and structural analysis of biologically active compounds: applications in proteomics, biomarkers, synthesis, isolation, and characterization of natural products, studies of redox systems in food and enzymatic synthesis
• Grantee: Emanuel Carrilho
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 16/07137-0 - Proteomic analysis of Biomphalaria glabrata infected with Schistosoma mansoni and Angiostrongylus cantonensis
• Grantee: Silmara Marques Allegretti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/07364-9 - Investigation of posttranslational histone modifications and co-regulators of expression in the Schistosoma mansoni stages
• Grantee: Fernanda Janku Cabral
• Support Opportunities: Regular Research Grants