Determination of the optimum dietary essential amino acid profile for growing phase of Nile tilapia by deletion method

Author(s)

do Nascimento, Thiago M. T. ^[1] ; Mansano, Cleber F. M. ^[2] ; Peres, Helena ^[3] ; Rodrigues, Fabio H. F. ^[1] ; Khan, Kifayat U. ^[1] ; Romaneli, Rafael S. ^[1] ; Sakomura, Nilva K. ^[4] ; Fernandes, Joao B. K. ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Fac Agr & Vet Sci, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] Brazil Univ, Campus Fernandopolis, Est Projetada F-1 S-N, BR-15600000 Fernandopolis, SP - Brazil

^[3] Univ Porto, CIMAR CIIMAR Ctr Interdisciplinar Invest Marinha, Rua Bragas 289, P-4050123 Porto - Portugal

^[4] Univ Estadual Paulista UNESP, Fac Agr & Vet Sci, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

• Document type: Journal article
• Source: Aquaculture; v. 523, JUN 30 2020.
• Web of Science Citations: 1

Abstract

The present study aimed to determine the optimal ratios of essential amino acids (EAAs) for Nile tilapia (Oreochromis niloticus) juveniles (150-200 g) using the deletion method. A total of 880 Nile tilapia juveniles (158.78 +/- 0.9 g) were randomly distributed among 44 tanks (20 fish per tank/replicate) of 1500-L capacity each. During the 53 days feeding trial, fish were fed with the respective diets and daily feed intake was regularly recorded. In order to prevent any feed wastage, the fish were hand-fed three times a day until apparent satiation. A balanced diet (BD) and other ten diets were formulated by the deletion method, where the BD diet was adjusted to result in a reduction of 45% of each tested EAA. A group of fish at the beginning and at the end of the experiment was euthanized for the determination of the carcass nitrogen (N) composition. Based on the results of the present study, the optimal balance of essential amino acids for Nile tilapia juveniles weighing 150 and 200 g (g 16 g(-1) N) was estimated as, arginine 4.88, lysine 5.87, threonine 6.02, histidine 1.73, isoleucine 3.26, leucine 4.82, methionine 2.36, phenylalanine 3.66, valine 3.46 and tryptophan 0.87. (AU)

• FAPESP's process: 16/11016-3 - Determining in vivo fish body composition method by the use of non-invasive technique
• Grantee: Cleber Fernando Menegasso Mansano
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/06093-6 - Prediction model of tilapias and pacus nutritional requirements
• Grantee: Thiago Matias Torres Do Nascimento
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/25761-4 - Modeling production and nutritional requirements of poultry and fish
• Grantee: Nilva Kazue Sakomura
• Support Opportunities: Research Projects - Thematic Grants