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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mitofusin 1 is required for oocyte growth and communication with follicular somatic cells

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Author(s):
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Carvalho, Karen F. [1] ; Machado, Thiago S. [2] ; Garcia, Bruna M. [1] ; Zangirolamo, Amanda F. [3] ; Macabelli, Carolina H. [1] ; Sugiyama, Fabricia H. C. [1] ; Grejo, Mateus P. [1] ; Neto, J. Djaci Augusto [1] ; Tostes, Katiane [1] ; Ribeiro, Fernanda K. S. [1] ; Sarapiao, Fabiana D. [3] ; Pandey, Anand K. [1, 4] ; Nociti, Ricardo P. [5] ; Tizioto, Polyana [6] ; Coutinho, Luiz Lehman [7] ; Meirelles, Flavio V. [2, 5] ; Guimaraes, Francisco E. G. [8] ; Pernas, Lena [9] ; Seneda, Marcelo M. [3] ; Chiaratti, Marcos R. [2, 1]
Total Authors: 20
Affiliation:
[1] Univ Fed Sao Carlos, Departamento Genet & Evolucao, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Sao Paulo - Brazil
[3] Univ Estadual Londrina, Natl Inst Sci & Technol Dairy Prod Chain INCT LEI, Londrina, Parana - Brazil
[4] Lala Lajpat Rai Univ Vet & Anim Sci, Coll et Sci, Hisar, Haryana - India
[5] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Pirassununga - Brazil
[6] NGS Solucoes Genom, Piracicaba - Brazil
[7] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil
[8] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos - Brazil
[9] Max Planck Inst Biol Ageing, Cologne - Germany
Total Affiliations: 9
Document type: Journal article
Source: FASEB JOURNAL; v. 34, n. 6 APR 2020.
Web of Science Citations: 1
Abstract

Mitochondrial function, largely regulated by the dynamics of this organelle, is inextricably linked to the oocyte health. In comparison with most somatic cells, mitochondria in oocytes are smaller and rounder in appearance, suggesting limited fusion. The functional implications of this distinct morphology, and how changes in the mitochondrial shape translate to mitochondrial function in oogenesis is little understood. We, therefore, asked whether the pro-fusion proteins mitofusins 1 (MFN1) and 2 (MFN2) are required for the oocyte development. Here we show that oocyte-specific deletion of Mfn1, but not Mfn2, prevents the oocyte growth and ovulation due to a block in folliculogenesis. We pinpoint the loss of oocyte growth and ovulation to impaired PI3K-Akt signaling and disrupted oocyte-somatic cell communication. In support, the double loss of Mfn1 and Mfn2 partially rescues the impaired PI3K-Akt signaling and defects in oocyte development secondary to the single loss of Mfn1. Together, this work demonstrates that the mitochondrial function influences the cellular signaling during the oocyte development, and highlights the importance of distinct, nonredundant roles of MFN1 and MFN2 in oogenesis. (AU)

FAPESP's process: 16/11935-9 - Effect of the knockout of Mitofusin 2 on mitochondria, endoplasmic reticulum and mitophagy in murine oocytes
Grantee:Bruna Martins Garcia
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/50231-6 - Molecular basis of mitochondrial inheritance: the role of mitochondrial fusion
Grantee:Marcos Roberto Chiaratti
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
Grantee:Igor Polikarpov
Support type: Multi-user Equipment Program
FAPESP's process: 16/07868-4 - Effect of the mitofusins knockout on the inheritance of deleterious Mitochondrial DNA in mouse embryionic fibroblasts
Grantee:Carolina Habermann Macabelli
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/04372-0 - Mitochondrial DNA: mechanisms for genome integrity maintenance and impact on disease
Grantee:Nadja Cristhina de Souza Pinto
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/11942-5 - Fertility effect of the knockout of Mitofusin 1 on murine oocytes
Grantee:Karen Freire Carvalho
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC