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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Early Diagnosis of Alzheimer's Disease in Blood Using a Disposable Electrochemical Microfluidic Platform

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Author(s):
de Oliveira, Tassia R. [1] ; Erbereli, Camila R. [1] ; Manzine, Patricia R. [2] ; Magalhaes, Thamires N. C. [3] ; Balthazar, Marcio L. F. [3] ; Cominetti, Marcia R. [2] ; Faria, Ronaldo C. [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Gerontol, BR-13565905 Sao Carlos, SP - Brazil
[3] Univ Estadual Campinas, Dept Neurol, BR-13083872 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ACS SENSORS; v. 5, n. 4, p. 1010-1019, APR 24 2020.
Web of Science Citations: 7
Abstract

Alzheimer's disease (AD) is a neurodegenerative condition that affects a large number of elderly people worldwide and has a high social and economic impact. The diagnosis of AD in early stage can significantly improve the evolution and prognosis of the disease. We report the use of A Disintegrin And Metalloprotease 10 (ADAM10) as a blood biomarker for the early diagnosis of AD. A simple, low-cost, sensitive, and disposable microfluidic platform (D mu P) was developed for ADAM10 detection in plasma and cerebrospinal fluid based on electrochemical immunosensors. The assay was designed to accurately detect ADAM10 in serum, with a limit of detection of 0.35 fg/mL. ADAM10 was detected in subjects divided into cognitively healthy subjects, subjects with mild cognitive impairment, and AD patients in different disease stages. An increase in protein levels was found throughout the disease, and good D mu P accuracy in differentiating individuals was observed. The D mu P provided significantly better sensitivity than the well-established enzyme-linked immunosorbent assay test. ADAM10 and its detection using the D mu P were proven to be an alternative tool for the early diagnosis and monitoring of AD, bringing new exciting possibilities to improve the quality of life of AD patients. (AU)

FAPESP's process: 17/24839-0 - Nanoelectronics and nanoscale electrochemistry: fundaments and applications
Grantee:Paulo Roberto Bueno
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/24053-7 - Development of simple electrochemical methods for the detection of biomarkers for the diagnosis of diseases
Grantee:Ronaldo Censi Faria
Support type: Regular Research Grants
FAPESP's process: 15/26084-1 - Evaluation of platelet ADAM10 in non-Alzheimer's dementias
Grantee:Patricia Regina Manzine Moralles
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/15571-7 - Mapping the progression of subjective cognitive decline to mild cognitive impairment and Alzheimer´s disease dementia with multimodal biomarkers
Grantee:Marcio Luiz Figueredo Balthazar
Support type: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 14/22401-0 - DEVELOPMENT OF DISPOSABLE IMMUNOSENSORS FOR DETECTION OF ALZHEIMER'S DISEASE BIOMARKERS
Grantee:Tássia Regina de Oliveira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/19890-1 - Development of methods for diagnosis of diseases using magnetic particles and an array of imunossensors in a disposable microfluidic system
Grantee:Ronaldo Censi Faria
Support type: Regular Research Grants