Ciliary melanin-concentrating hormone receptor 1 (MCHR1) is widely distributed in the murine CNS in a sex-independent manner

Author(s):Show less -

Diniz, Giovanne B. ^{[1, 2]} ; Battagello, Daniella S. ^{[1, 3]} ; Klein, Marianne O. ^{[1, 3]} ; Bono, Bianca S. M. ^[4] ; Ferreira, Jozelia G. P. ^{[1, 3]} ; Motta-Teixeira, Livia C. ^{[1, 3]} ; Duarte, Jessica C. G. ^{[1, 3]} ; Presse, Francoise ^[5] ; Nahon, Jean-Louis ^[5] ; Adamantidis, Antoine ^[6] ; Chee, Melissa J. ^[4] ; Sita, Luciane V. ^{[1, 3]} ; Bittencourt, Jackson C. ^{[1, 7, 3]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo, SP - Brazil

^[2] Yale Sch Med, Dept Neurosurg, New Haven, CT - USA

^[3] Bittencourt, Jackson C., Univ Sao Paulo, Inst Psychol, Ctr Neurosci \& Behav, Sao Paulo, SP, Brazil.Diniz, Giovanne B., Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo, SP - Brazil

^[4] Carleton Univ, Dept Neurosci, Ottawa, ON - Canada

^[5] Univ Cote Azur, Inst Pharmacol Mol & Cellulaire IPMC, CNRS, Valbonne - France

^[6] Univ Bern, Dept Neurol, Inselspital, Bern - Switzerland

^[7] Univ Sao Paulo, Inst Psychol, Ctr Neurosci & Behav, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Journal of Neuroscience Research; v. 98, n. 10 JUN 2020.
• Web of Science Citations: 0

Abstract

Melanin-concentrating hormone (MCH) is a ubiquitous vertebrate neuropeptide predominantly synthesized by neurons of the diencephalon that can act through two G protein-coupled receptors, called MCHR1 and MCHR2. The expression ofMchr1has been investigated in both rats and mice, but its synthesis remains poorly described. After identifying an antibody that detects MCHR1 with high specificity, we employed immunohistochemistry to map the distribution of MCHR1 in the CNS of rats and mice. Multiple neurochemical markers were also employed to characterize some of the neuronal populations that synthesize MCHR1. Our results show that MCHR1 is abundantly found in a subcellular structure called the primarycilium, which has been associated, among other functions, with the detection of free neurochemical messengers present in the extracellular space. Ciliary MCHR1 was found in a wide range of areas, including the olfactory bulb, cortical mantle,striatum, hippocampal formation, amygdala, midline thalamic nuclei, periventricular hypothalamic nuclei, midbrain areas, and in the spinal cord. No differences were observed between male and female mice, and interspecies differences were found in the caudate-putamen nucleus and the subgranular zone. Ciliary MCHR1 was found in close association with several neurochemical markers, including tyrosine hydroxylase, calretinin, kisspeptin, estrogen receptor, oxytocin, vasopressin, and corticotropin-releasing factor. Given the role of neuronal primaryciliain sensing free neurochemical messengers in the extracellular fluid, the widespread distribution of ciliary MCHR1, and the diverse neurochemical populations who synthesize MCHR1, our data indicate that nonsynaptic communication plays a prominent role in the normal function of the MCH system. (AU)

• FAPESP's process: 15/05990-4 - The melanin-concentrating hormone [MCH] peptidergic system: neurogenesis and lactation
• Grantee: Jozélia Gomes Pacheco Ferreira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/02748-0 - Study of the anatomical basis for a volume transmission mechanism on the Melanin Concentrating Hormone [MCH] peptidergic system
• Grantee: Giovanne Baroni Diniz
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 17/16293-8 - The existence of neurogenesis during the lactation period - multiuser equipment approved in Fapesp Grant number 2016/02224-1: confocal microscope, brand Zeiss, model LSM 800
• Grantee: Jackson Cioni Bittencourt
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 17/17998-5 - Evaluating chronic administration of melanin concentrating hormone (MCH) effects in lactating Long-Evans rats
• Grantee: Marianne Orlandini Klein
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/18941-4 - Effects of maternal melatonin deprivation during pregnancy and lactation on the offspring neuroimmune system
• Grantee: Lívia Clemente Motta Teixeira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/19845-9 - CHARACTERIZATION OF THE MELANIN-CONCENTRATING HORMONE RECEPTOR 1 (MCHR1) IN THE BRAIN, MAMMARY GLAND, UTERUS AND OVARIES IN WILD TYPE AND MCHR1 KNOCKOUT MICE.
• Grantee: Jozélia Gomes Pacheco Ferreira
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 16/02224-1 - The existence of neurogenesis during the lactation period
• Grantee: Jackson Cioni Bittencourt
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/22313-3 - Influence of the absence of maternal melatonin during pregnancy and lactation on neurogenesis and on somatic and sensorimotor development of the offspring.
• Grantee: Lívia Clemente Motta Teixeira
• Support Opportunities: Scholarships in Brazil - Doctorate