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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genomic insights into multidrug-resistant and hypervirulent Klebsiella pneumoniae co-harboring metal resistance genes in aquatic environments

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Author(s):
Rueda Furlan, Joao Pedro [1] ; Savazzi, Eduardo Angelino [2] ; Stehling, Eliana Guedes [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto - Brazil
[2] Companhia Ambiental Estado Sao Paulo CETESB, Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 201, SEP 15 2020.
Web of Science Citations: 0
Abstract

Klebsiella pneumoniae is one of the most important pathogens related to hospital-acquired infections. The incidence of infections by hypervirulent K. pneumoniae (hvKp), especially community-acquired infections, has been increasing in recent decades. The occurrence of multidrug-resistant (MDR) hvKp has been increasingly reported worldwide decreasing the treatment options, which is a concern. Aquatic environments have been considered a reservoir of MDR pathogens, which contribute to the spread of MDR pathogens. Therefore, this study aimed to characterize MDR-hvKp strains obtained from public aquatic environments using whole genome sequencing in Brazil. Resistome analysis showed ARGs to beta-lactams, quinolones, tetracyclines, sulfonamides, and fosfomycin as well as several metal resistance genes. Virulome analysis showed several virulence genes. Besides, genomic islands, CRISPR and prophage-related sequences were also detected. MLST analysis revealed the presence of two novel sequences types (STs) belonging to different clonal complexes (CCs) {[}ST4415 (CC515) and ST4416 (CC2654)], and one already described {[}ST661 (CC661)]. The presence of MDR-hvKp lineages in water sources belonging to STs and CCs associated with humans and animals shows the ability of these pathogens to spread to different aquatic environments. This study reports for the first time two novel STs of MDR-hvKp as well as the presence of a rare ST661 closely related to outbreaks in aquatic environments, and contributes to surveillance studies and MDR-hvKp monitoring worldwide. (AU)

FAPESP's process: 18/19539-0 - Molecular characterization of Escherichia coli isolates from the environment
Grantee:Eliana Guedes Stehling
Support Opportunities: Regular Research Grants