Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids

Author(s)

Kurnik, Isabelle S. ^[1] ; Noronha, Mariana A. ^[2] ; Camara, Mayra C. C. ^[2] ; Mazzola, Priscila G. ^[2] ; Vicente, Antonio A. ^[3] ; Pereira, Jorge F. B. ^{[1, 4]} ; Lopes, Andre M. ^[2]

Affiliation

^[1] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Engn Bioproc & Biotechnol, UNESP, Araraquara, SP - Brazil

^[2] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas, SP - Brazil

^[3] Univ Minho, Ctr Biol Engn CEB, Campus Gualtar, P-4710057 Braga - Portugal

^[4] Univ Coimbra, Dept Chem Engn, CIEPQPF, Rua Silvio Lima, Polo 2 Pinhal Marrocos, P-3030790 Coimbra - Portugal

• Document type: Journal article
• Source: Separation and Purification Technology; v. 250, NOV 1 2020.
• Web of Science Citations: 0

Abstract

Novel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). CCM stability in the presence of ATPMS components was also evaluated. CCM is stable up to 24 h in copolymer (1.0 - 10.0 wt%) and ILs (0.1 - 3.0 M) aqueous solutions. Very mild phase separation conditions (close to room temperature) were achieved by adding cholinium ILs to the Pluronic F68 + Mcllvaine buffer at pH 6.0 solution. The decrease of cloud-point temperature is dependent on the relative hydrophobicity of IL anion, {[}Hex](-) > {[}But](-) > {[}Pro](-) > {[}Ac](-) > Cl-. ATPMS composed of more hydrophobic ILs ({[}Ch] {[}Hex] > {[}Ch] {[}But] > {[}Ch] {[}Pro]) are most efficient in the partition of commercial CCM into polymeric micelles (PMs)-rich phase. The best ATPMS (0.70 M {[}Ch] {[}But] and 0.60 M {[}Ch] {[}Hex]-based ATPMS) were then used to purify CCM from a crude extract of Curcuma longa L. Both systems were very selective to separate CCM from protein-based contaminants (selectivity values >= 25; purification yields >= 12-fold). Pluronic F68-based ATPMS are promising for selective separation of hydrophobic biomolecules by using cholinium-based ILs as adjuvants to adjust phase separation temperatures and biomolecules' partition. (AU)

• FAPESP's process: 14/16424-7 - Optimization and scale-up of liquid-liquid extraction process with ionic liquids (ILs) as a sustainable tool for the separation of the anti-leukemia biopharmaceutical L-asparaginase (ASPase)
• Grantee: Jorge Pereira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/10789-1 - Combinatorial therapy using polymersomes decorated with transferrin and incorporated into chitosan hydrogels as smart drug delivery systems for melanoma tumor cells
• Grantee: André Moreni Lopes
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/05624-9 - Encapsulation and in vitro release studies of curcumin in polymeric micelles composed of PEO-PPO-PEO triblock copolymers
• Grantee: Isabelle Souza Kurnik
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• FAPESP's process: 18/05111-9 - Aqueous two-phase micellar systems based on ionic liquids as platforms for the curcumin encapsulation in polymeric micelles
• Grantee: Isabelle Souza Kurnik
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/08549-8 - Polymersomes decorated with transferrin and evaluation of their stability and drug release behavior
• Grantee: Mayra Caroline Cunha Câmara
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/10799-0 - Combinatorial therapy using polymersomes decorated with transferrin and incorporated into chitosan hydrogels as smart drug delivery systems for melanoma tumor cells
• Grantee: André Moreni Lopes
• Support Opportunities: Scholarships in Brazil - Young Researchers