| Full text | |
| Author(s): |
Miserani Magalhaes, Rubens Daniel
[1, 2, 3]
;
Mattos, Eliciane Cevolani
[1, 4]
;
Rozanski, Andrei
[2]
;
Favoretto Galante, Pedro Alexandre
[2]
;
Palmisano, Giuseppe
[5]
;
Cruz, Angela Kaysel
[3]
;
Colli, Walter
[1]
;
Camargo, Anamaria Aranha
[2]
;
Manso Alves, Maria Julia
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil
[2] Hosp Sirio Libanes, Ctr Oncol Mol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo - Brazil
[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | PLoS Neglected Tropical Diseases; v. 14, n. 5 MAY 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Adhesion of T. cruzi trypomastigotes to components of the extracellular matrix (ECM) is an important step in mammalian host cell invasion. We have recently described a significant increase in the tyrosine nitration levels of histones H2A and H4 when trypomastigotes are incubated with components of the ECM. In this work, we used chromatin immunoprecipitation (ChIP) with an anti-nitrotyrosine antibody followed by mass spectrometry to identify nitrated DNA binding proteins in T. cruzi and to detect alterations in nitration levels induced upon parasite incubation with the ECM. Histone H1, H2B, H2A and H3 were detected among the 9 most abundant nitrated DNA binding proteins using this proteomic approach. One nitrated tyrosine residue (Y29) was identified in Histone H2B in the MS/MS spectrum. In addition, we observed a significant increase in the nitration levels of histones H1, H2B, H2A and H4 upon parasite incubation with ECM. Finally, we used ChIP-Seq to map global changes in the DNA binding profile of nitrated proteins. We observed a significant change in the binding pattern of nitrated proteins to DNA after parasite incubation with ECM. This work provides the first global profile of nitrated DNA binding proteins in T. cruzi and additional evidence for modification in the nitration profile of histones upon parasite incubation with ECM. Our data also indicate that the parasite interaction with the ECM induces alterations in chromatin structure, possibly affecting nuclear functions. (AU) | |
| FAPESP's process: | 14/10046-0 - Involvement of NO-dependent modifications in the control of Trypanosoma cruzi gene transcription throughout the adhesion of the parasite to the host extracellular matrix |
| Grantee: | Rubens Daniel Miserani Magalhães |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/16002-2 - Identification of flagellar proteins from Trypanosoma cruzi modified due to the parasite adhesion to the host extracelullar matrix and cytoskeleton. |
| Grantee: | Eliciane Cevolani Mattos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 14/25494-9 - Trypanosoma Cruzi signaling due to changes in the external conditions: ECM and pH changes |
| Grantee: | Maria Julia Manso Alves |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Research Grants - Young Investigators Grants |