The Rat Mammary Gland as a Novel Site of Expression of Melanin-Concentrating Hormone Receptor 1 mRNA and Its Protein Immunoreactivity

Author(s):Show less -

Battagello, Daniella S. ^{[1, 2]} ; Lorenzon, Aline R. ^[3] ; Diniz, Giovanne B. ^[2] ; Motta-Teixeira, Livia C. ^[4] ; Klein, Marianne O. ^[2] ; Ferreira, Jozelia G. P. ^[2] ; Arias, Carlos M. ^[5] ; Adamantidis, Antoine ^[6] ; Sita, V, Luciane ; Cipolla-Neto, Jose ^[4] ; Bevilacqua, Estela M. A. F. ^[3] ; Sawchenko, Paul E. ^[5] ; Bittencourt, Jackson C. ^{[1, 7]}

Affiliation

^[1] Univ Sao Paulo, Nucleo Neurociencias & Comportamento, Inst Psicol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Neuroanat Ouim, Inst Ciencias Biomed, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Biol Celular & Desenvolvimento, Inst Ciencias Biomed, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Fisiol & Biofis, Inst Ciencias Biomed, Sao Paulo - Brazil

^[5] Salk Inst Biol Studies, Lab Neuronal Struct & Funct, 10010 N Torrey Pines Rd, La Jolla, CA 92037 - USA

^[6] Univ Bern, Dept Neurol, Inselspital, Bern - Switzerland

^[7] Sita, Luciane, V, Univ Sao Paulo, Lab Neuroanat Ouim, Inst Ciencias Biomed, Sao Paulo - Brazil

• Document type: Journal article
• Source: FRONTIERS IN ENDOCRINOLOGY; v. 11, JUL 31 2020.
• Web of Science Citations: 0

Abstract

Lactation is a complex physiological process, depending on orchestrated central and peripheral events, including substantial brain plasticity. Among these events is a novel expression of pro-melanin-concentrating hormone (Pmch) mRNA in the rodent hypothalamus, such as the ventral part of the medial preoptic area (vmMPOA). This expression reaches its highest levels around postpartum day 19 (PPD19), when dams transition from lactation to the weaning period. The appearance of this lactation-relatedPmchexpression occurs simultaneously with the presence of one of thePmchproducts, melanin-concentrating hormone (MCH), in the serum. Given the relevance of the MPOA to maternal physiology and the contemporaneity betweenPmchexpression in this structure and the weaning period, we hypothesized that MCH has a role in the termination of lactation, acting as a mediator between central and peripheral changes. To test this, we investigated the presence of the MCH receptor 1 (MCHR1) and its gene expression in the mammary gland of female rats in different stages of the reproductive cycle. To that end,in situhybridization, RT-PCR, RT-qPCR, nucleotide sequencing, immunohistochemistry, and Western blotting were employed. AlthoughMchr1expression was detected in the epidermis and dermis of both diestrus and lactating rats, parenchymal expression was exclusively found in the functional mammary gland of lactating rats. The expression ofMchr1mRNA oscillated through the lactation period and reached its maximum in PPD19 dams. Presence of MCHR1 was confirmed with immunohistochemistry with preferential location of MCHR1 immunoreactive cells in the alveolar secretory cells. As was the case for gene expression, the MCHR1 protein levels were significantly higher in PPD19 than in other groups. Our data demonstrate the presence of an anatomical basis for the participation of MCH peptidergic system on the control of lactation through the mammary gland, suggesting that MCH could modulate a prolactation action in early postpartum days and the opposite role at the end of the lactation. (AU)

• FAPESP's process: 15/05990-4 - The melanin-concentrating hormone [MCH] peptidergic system: neurogenesis and lactation
• Grantee: Jozélia Gomes Pacheco Ferreira
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 16/02748-0 - Study of the anatomical basis for a volume transmission mechanism on the Melanin Concentrating Hormone [MCH] peptidergic system
• Grantee: Giovanne Baroni Diniz
• Support type: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 17/16293-8 - The existence of neurogenesis during the lactation period - multiuser equipment approved in Fapesp Grant number 2016/02224-1: confocal microscope, brand Zeiss, model LSM 800
• Grantee: Jackson Cioni Bittencourt
• Support type: Multi-user Equipment Program
• FAPESP's process: 10/52068-0 - The putative envolvement of melanin-concentrating hormone in the control of lactation
• Grantee: Jackson Cioni Bittencourt
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 16/04210-8 - Sdf2 factor in the activation of the Unfolded Protein Response (UPR) signaling network in the trophoblast physiology and pathology
• Grantee: Estela Maris Andrade Forell Bevilacqua
• Support type: Regular Research Grants
• FAPESP's process: 17/17998-5 - Evaluating chronic administration of melanin concentrating hormone (MCH) effects in lactating Long-Evans rats
• Grantee: Marianne Orlandini Klein
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 14/50457-0 - Melatonin and the control of energy metabolism: basic, clinical and epidemiological research
• Grantee: José Cipolla Neto
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 12/04554-8 - IDENTIFICATION, MOLECULAR CHARACTERIZATION, MAPPING AND COLOCALIZATION OF THE RECEPTOR 1 MELANIN-CONCENTRATING HORMONE IN MAMMARY GLAND OF LACTATING AND NON-LACTATING RATS.
• Grantee: Daniella Sabino Battagello
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/13052-1 - The role of Sdf2 (stromal cell derived factor 2) in cell survival/apoptosis of trophoblast cells via endoplasmic reticulum stress
• Grantee: Aline Rodrigues Lorenzon
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 16/18941-4 - Effects of maternal melatonin deprivation during pregnancy and lactation on the offspring neuroimmune system
• Grantee: Lívia Clemente Motta Teixeira
• Support type: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/19845-9 - CHARACTERIZATION OF THE MELANIN-CONCENTRATING HORMONE RECEPTOR 1 (MCHR1) IN THE BRAIN, MAMMARY GLAND, UTERUS AND OVARIES IN WILD TYPE AND MCHR1 KNOCKOUT MICE.
• Grantee: Jozélia Gomes Pacheco Ferreira
• Support type: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 16/02224-1 - The existence of neurogenesis during the lactation period
• Grantee: Jackson Cioni Bittencourt
• Support type: Research Projects - Thematic Grants