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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Rat Mammary Gland as a Novel Site of Expression of Melanin-Concentrating Hormone Receptor 1 mRNA and Its Protein Immunoreactivity

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Battagello, Daniella S. [1, 2] ; Lorenzon, Aline R. [3] ; Diniz, Giovanne B. [2] ; Motta-Teixeira, Livia C. [4] ; Klein, Marianne O. [2] ; Ferreira, Jozelia G. P. [2] ; Arias, Carlos M. [5] ; Adamantidis, Antoine [6] ; Sita, V, Luciane ; Cipolla-Neto, Jose [4] ; Bevilacqua, Estela M. A. F. [3] ; Sawchenko, Paul E. [5] ; Bittencourt, Jackson C. [1, 7]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Nucleo Neurociencias & Comportamento, Inst Psicol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Lab Neuroanat Ouim, Inst Ciencias Biomed, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Biol Celular & Desenvolvimento, Inst Ciencias Biomed, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Fisiol & Biofis, Inst Ciencias Biomed, Sao Paulo - Brazil
[5] Salk Inst Biol Studies, Lab Neuronal Struct & Funct, 10010 N Torrey Pines Rd, La Jolla, CA 92037 - USA
[6] Univ Bern, Dept Neurol, Inselspital, Bern - Switzerland
[7] Sita, Luciane, V, Univ Sao Paulo, Lab Neuroanat Ouim, Inst Ciencias Biomed, Sao Paulo - Brazil
Total Affiliations: 7
Document type: Journal article
Source: FRONTIERS IN ENDOCRINOLOGY; v. 11, JUL 31 2020.
Web of Science Citations: 0
Abstract

Lactation is a complex physiological process, depending on orchestrated central and peripheral events, including substantial brain plasticity. Among these events is a novel expression of pro-melanin-concentrating hormone (Pmch) mRNA in the rodent hypothalamus, such as the ventral part of the medial preoptic area (vmMPOA). This expression reaches its highest levels around postpartum day 19 (PPD19), when dams transition from lactation to the weaning period. The appearance of this lactation-relatedPmchexpression occurs simultaneously with the presence of one of thePmchproducts, melanin-concentrating hormone (MCH), in the serum. Given the relevance of the MPOA to maternal physiology and the contemporaneity betweenPmchexpression in this structure and the weaning period, we hypothesized that MCH has a role in the termination of lactation, acting as a mediator between central and peripheral changes. To test this, we investigated the presence of the MCH receptor 1 (MCHR1) and its gene expression in the mammary gland of female rats in different stages of the reproductive cycle. To that end,in situhybridization, RT-PCR, RT-qPCR, nucleotide sequencing, immunohistochemistry, and Western blotting were employed. AlthoughMchr1expression was detected in the epidermis and dermis of both diestrus and lactating rats, parenchymal expression was exclusively found in the functional mammary gland of lactating rats. The expression ofMchr1mRNA oscillated through the lactation period and reached its maximum in PPD19 dams. Presence of MCHR1 was confirmed with immunohistochemistry with preferential location of MCHR1 immunoreactive cells in the alveolar secretory cells. As was the case for gene expression, the MCHR1 protein levels were significantly higher in PPD19 than in other groups. Our data demonstrate the presence of an anatomical basis for the participation of MCH peptidergic system on the control of lactation through the mammary gland, suggesting that MCH could modulate a prolactation action in early postpartum days and the opposite role at the end of the lactation. (AU)

FAPESP's process: 15/05990-4 - The melanin-concentrating hormone [MCH] peptidergic system: neurogenesis and lactation
Grantee:Jozélia Gomes Pacheco Ferreira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/02748-0 - Study of the anatomical basis for a volume transmission mechanism on the Melanin Concentrating Hormone [MCH] peptidergic system
Grantee:Giovanne Baroni Diniz
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/16293-8 - The existence of neurogenesis during the lactation period - multiuser equipment approved in Fapesp Grant number 2016/02224-1: confocal microscope, brand Zeiss, model LSM 800
Grantee:Jackson Cioni Bittencourt
Support type: Multi-user Equipment Program
FAPESP's process: 10/52068-0 - The putative envolvement of melanin-concentrating hormone in the control of lactation
Grantee:Jackson Cioni Bittencourt
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/04210-8 - Sdf2 factor in the activation of the Unfolded Protein Response (UPR) signaling network in the trophoblast physiology and pathology
Grantee:Estela Maris Andrade Forell Bevilacqua
Support type: Regular Research Grants
FAPESP's process: 17/17998-5 - Evaluating chronic administration of melanin concentrating hormone (MCH) effects in lactating Long-Evans rats
Grantee:Marianne Orlandini Klein
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/50457-0 - Melatonin and the control of energy metabolism: basic, clinical and epidemiological research
Grantee:José Cipolla Neto
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/04554-8 - IDENTIFICATION, MOLECULAR CHARACTERIZATION, MAPPING AND COLOCALIZATION OF THE RECEPTOR 1 MELANIN-CONCENTRATING HORMONE IN MAMMARY GLAND OF LACTATING AND NON-LACTATING RATS.
Grantee:Daniella Sabino Battagello
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/13052-1 - The role of Sdf2 (stromal cell derived factor 2) in cell survival/apoptosis of trophoblast cells via endoplasmic reticulum stress
Grantee:Aline Rodrigues Lorenzon
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/18941-4 - Effects of maternal melatonin deprivation during pregnancy and lactation on the offspring neuroimmune system
Grantee:Lívia Clemente Motta Teixeira
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 16/19845-9 - CHARACTERIZATION OF THE MELANIN-CONCENTRATING HORMONE RECEPTOR 1 (MCHR1) IN THE BRAIN, MAMMARY GLAND, UTERUS AND OVARIES IN WILD TYPE AND MCHR1 KNOCKOUT MICE.
Grantee:Jozélia Gomes Pacheco Ferreira
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 16/02224-1 - The existence of neurogenesis during the lactation period
Grantee:Jackson Cioni Bittencourt
Support type: Research Projects - Thematic Grants