Bioaccessibility and cellular uptake by Caco-2 cells of carotenoids and chlorophylls from orange peels: A comparison between conventional and ionic liquid mediated extractions

Author(s)

Murador, Daniella C. ^[1] ; Mesquita, Leonardo M. De Souza ^[1] ; Neves, V, Bruna ; Braga, Anna Rc ^{[2, 3]} ; Martins, Paula L. G. ^[4] ; Zepka, Leila Q. ^[5] ; De Rosso, Veridiana V. ^[2]

Affiliation

^[1] Univ Fed Sao Paulo UNIFESP, Dept Biosci, Rua Silva Jardim 136, BR-11015020 Santos, SP - Brazil

^[2] Neves, Bruna, V, Univ Fed Sao Paulo UNIFESP, Dept Biosci, Rua Silva Jardim 136, BR-11015020 Santos, SP - Brazil

^[3] Fed Inst Sao Paulo, Av Clara Gianotti de Souza 5180, BR-11900000 Registro - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Dept Chem Engn, Rua Sao Nicolau 201, BR-09972270 Diadema, SP - Brazil

^[5] Fed Univ Santa Maria UFSM, Dept Food Sci & Technol, BR-97105900 Santa Maria, RS - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 339, MAR 1 2021.
• Web of Science Citations: 1

Abstract

Native extracts from orange peels were obtained by a conventional method using acetone and, an alternative method using ionic liquid (1-butyl-3-methylimidazolium chloride ({[}C(4)mim]Cl)). The bioaccessibilities and cellular uptakes of carotenoids, esters and chlorophylls were evaluated, since the influence of esterification on bioaccessibility and bioavailability is not well established. For this, the extracts were emulsified, submitted to in vitro simulated digestion model according to the INFOGEST protocol, followed by uptake by Caco-2 cells. Compounds were separated, identified and quantified by HPLC-PDA-MS/MS. After digestion, 22.0% and 26.2% of the total carotenoids and 45.9% and 68.7% of the chlorophylls were bioaccessible from the acetone and {[}C(4)mim]Cl extracts, respectively. The bioaccessibilities of xanthophylls and carotenes were significantly higher than those of the mono- and diesters. The uptake by Caco-2 cells varied from 130.2 to 131.9 ng/mg cell protein for total carotenoids and from 243.8 to 234.2 ng/mg cell protein for chlorophylls in the acetone and {[}C(4)mim]Cl extracts, respectively. In general, xanthophylls and esters were better absorbed than carotenes. (AU)

• FAPESP's process: 15/26789-5 - Ionic Liquids As Green Extractors for Achievement of Carotenoids from Orange Peel
• Grantee: Daniella Carisa Murador
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/18910-1 - Ionic Liquids as Green Extractors for Achievement of Carotenoids: Study of Bioavailability and Antioxidant Activit
• Grantee: Veridiana Vera de Rosso
• Support Opportunities: Regular Research Grants