Probiotic supplementation in marathonists and its impact on lymphocyte population and function after a marathon: a randomized placebo-controlled double-blind study

Author(s):Show less -

Batatinha, Helena ^{[1, 2]} ; Tavares-Silva, Edgar ^[3] ; Leite, Geovana S. F. ^[4] ; Resende, Ayane S. ^[4] ; Albuquerque, Jose A. T. ^[5] ; Arslanian, Christina ^[5] ; Fock, Ricardo A. ^[6] ; Lancha, Jr., Antonio H. ^[4] ; Lira, Fabio S. ^[7] ; Krueger, Karsten ^[8] ; Thomatieli-Santos, Ronaldo ^{[9, 3]} ; Rosa-Neto, Jose C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Immunometab Res Grp, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Cell & Dev Biol, 1524 Prof Lineu Prestes Ave, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Programa Posgrad Psicobiol, Santos, SP - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sports, Lab Appl Nutr & Metab, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

^[7] Univ Estadual Paulista UNESP, Sch Technol & Sci, Dept Phys Educ, Postgrad Program Movement Sci, Exercise & Immunome, Presidente Prudente - Brazil

^[8] Justus Liebig Univ Giessen, Dept Exercise Physiol & Sports Therapy, Giessen - Germany

^[9] Univ Fed Sao Paulo, Dept Biosci, Santos, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 10, n. 1 NOV 2 2020.
• Web of Science Citations: 0

Abstract

Probiotic supplementation arises as playing an immune-stimulatory role. High-intensity and -volume exercise can inhibit immune cell function, which threatens athletic performance and recovery. We hypothesized that 30 days of probiotic supplementation could stabilize the immune system of athletes preventing immune suppression after a marathon race. Twenty-seven male marathonists were double-blinded randomly into probiotic (Bifidobacterium-animalis-subsp.-Lactis (10x10(9)) and Lactobacillus-Acidophilus (10x10(9))+5 g of maltodextrin) and placebo (5 g of maltodextrin) group. They received 30 sachets and supplemented 1 portion/day during 30 days before the race. Blood were collected 30 days before (rest), 1 day before (pre), 1 h after (post) and 5 days after the race (recovery). Both chronic and acute exercise modulated a different T lymphocyte population (CD3(+)CD4(-)CD8(-) T-cells), increasing pre-race, decreasing post and returning to rest values at the recovery. The total number of CD8 T cell and the memory subsets statistically decreased only in the placebo group post-race. Pro-inflammatory cytokine production by stimulated lymphocytes decreased in the probiotic group after the supplementation period. 30 days of probiotic supplementation maintained CD8 T cell and effector memory cell population and played an immunomodulatory role in stimulated lymphocytes. Both, training and marathon modulated a non-classical lymphocyte population regardless of probiotic supplementation. (AU)

• FAPESP's process: 19/09854-9 - Effect of doxorubicin upon the adipose tissue: elucidation the role of PPARs family
• Grantee: José Cesar Rosa Neto
• Support type: Regular Research Grants
• FAPESP's process: 16/10561-8 - Probiotic Supplementation effect on oral microbiota modulation and upper respiratory tract infection in runners
• Grantee: Helena Angélica Pereira Batatinha
• Support type: Scholarships in Brazil - Doctorate