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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Size Matters: An Evaluation of the Molecular Basis of Ontogenetic Modifications in the Composition of Bothrops jararacussu Snake Venom

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Author(s):
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Freitas-de-Sousa, Luciana A. [1] ; Nachtigall, Pedro G. [2] ; Fortes-Junior, Jose A. [3] ; Holding, Matthew L. [4] ; Nystrom, Gunnar S. [4] ; Ellsworth, Schyler A. [4] ; Guimaries, Noranathan C. [1] ; Tioyama, Emilly [1] ; Ortiz, Flora [3] ; Silva, Bruno R. [3] ; Kunz, Tobias S. [3] ; Junqueira-de-Azevedo, Inicio L. M. [2] ; Grazziotin, Felipe G. [3] ; Rokyta, Darin R. [4] ; Moura-da-Silva, Ana M. [1, 5]
Total Authors: 15
Affiliation:
[1] Inst Butantan, Lab Imunopatol, Programa Posgrad Ciencias Toxinol, BR-05503900 Sao Paulo, SP - Brazil
[2] Inst Butantan, Lab Especial Toxinol Aplicada, BR-05503900 Sao Paulo, SP - Brazil
[3] Inst Butantan, Lab Colecoes Zool, BR-05503900 Sao Paulo, SP - Brazil
[4] Florida State Univ, Dept Biol Sci, Tallahassee, FL 32306 - USA
[5] Fundacao Med Trop Dr Heitor Vieira Dourado, Inst Pesquisa Clin Carlos Borborema, BR-69040000 Manaus, Amazonas - Brazil
Total Affiliations: 5
Document type: Journal article
Source: TOXINS; v. 12, n. 12 DEC 2020.
Web of Science Citations: 0
Abstract

Ontogenetic changes in venom composition have been described in Bothrops snakes, but only a few studies have attempted to identify the targeted paralogues or the molecular mechanisms involved in modifications of gene expression during ontogeny. In this study, we decoded B. jararacussu venom gland transcripts from six specimens of varying sizes and analyzed the variability in the composition of independent venom proteomes from 19 individuals. We identified 125 distinct putative toxin transcripts, and of these, 73 were detected in venom proteomes and only 10 were involved in the ontogenetic changes. Ontogenetic variability was linearly related to snake size and did not correspond to the maturation of the reproductive stage. Changes in the transcriptome were highly predictive of changes in the venom proteome. The basic myotoxic phospholipases A(2) (PLA(2)s) were the most abundant components in larger snakes, while in venoms from smaller snakes, PIII-class SVMPs were the major components. The snake venom metalloproteinases (SVMPs) identified corresponded to novel sequences and conferred higher pro-coagulant and hemorrhagic functions to the venom of small snakes. The mechanisms modulating venom variability are predominantly related to transcriptional events and may consist of an advantage of higher hematotoxicity and more efficient predatory function in the venom from small snakes. (AU)

FAPESP's process: 18/25749-8 - Study on the variability of venom in snakes of the Bothrops jararaca complex and its correlation with the processes of speciation in continental islands
Grantee:José Antonio Portes Junior
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/08589-7 - Mechanisms involved in the phenotype variability of venoms from snakes from the same group (Bothrops)
Grantee:Luciana Aparecida Freitas de Sousa
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 18/15753-8 - Proteolytic enzymes and phospholipases in snake venoms: distribution and mechanisms of action related to venom toxicity and the digestion of prey
Grantee:Noranathan da Costa Guimarães
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/26520-4 - Characterization of the interrelationship between transcriptomes, miRNomes and proteomes from the venom glands of Bothrops fonsecai and Bothrops cotiara
Grantee:Pedro Gabriel Nachtigall
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/50127-5 - Dimensions US-BIOTA São Paulo: scales of biodiversity: integrated studies of snake venom evolution and function across multiple levels of diversity
Grantee:Inácio de Loiola Meirelles Junqueira de Azevedo
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 17/24546-3 - Scales of biodiversity: integrated studies of snake evolution and venom function
Grantee:Luciana Aparecida Freitas de Sousa
Support Opportunities: Scholarships in Brazil - Post-Doctoral