Structure and Thermotropic Behavior of Bovine- and Porcine-Derived Exogenous Lung Surfactants

Author(s):Show less -

Oseliero Filho, Pedro Leonidas ^[1] ; Gerbelli, Barbara Bianca ^[2] ; Fornasier, Franccesca ^[3] ; Chaves Filho, Adriano B. ^[4] ; Yoshinaga, Marcos Yukio ^[4] ; Miyamoto, Sayuri ^[4] ; Mortara, Laura ^[4] ; Lacerda, Caroline Dutra ^[4] ; Cuccovia, Iolanda Midea ^[4] ; Pimentel, Andre Silva ^[3] ; Pinto Oliveira, Cristiano Luis ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Fis, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil

^[3] Pontiicia Univ Catolica Rio de Janeiro, Dept Quim, BR-22453900 Rio De Janeiro, RJ - Brazil

^[4] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Langmuir; v. 36, n. 48, p. 14514-14529, DEC 8 2020.
• Web of Science Citations: 0

Abstract

Two commercial exogenous pulmonary surfactants, Curosurf and Survanta, are investigated. Their thermotropic behavior and associated structural changes for the samples in bulk are characterized and described. For Survanta, the obtained results of differential scanning calorimetry showed a thermogram with three peaks on heating and only a single peak on cooling. Curosurf on the other hand, presents calorimetric thermograms with only one peak in both the heating and cooling scans. This distinct thermotropic behavior between the two pulmonary surfactants, a consequence of their particular compositions, is associated with structural changes that were evaluated by simultaneous small- and wide-angle X-ray scattering experiments with in situ temperature variation. Interestingly, for temperatures below similar to 35 degrees C for Curosurf and similar to 53 degrees C for Survanta, the scattering data indicated the coexistence of two lamellar phases with different carbon chain organizations. For temperatures above these limits, the coexistence of phases disappears, giving rise to a fluid phase in both pulmonary surfactants, with multilamelar vesicles for Curosurf and unilamellar vesicles for Survanta. This process is quasi-reversible under cooling, and advanced data analysis for the scattering data indicated differences in the structural and elastic properties of the pulmonary surfactants. The detailed and systematic investigation shown in this work expands on the knowledge of the structure and thermodynamic behavior of Curosurf and Survanta, being relevant from both physiological and biophysical perspectives and also providing a basis for further studies on other types of pulmonary surfactants. (AU)

• FAPESP's process: 16/24531-3 - Structural and biophysics properties of native and modified lipoproteins
• Grantee: Antonio Martins Figueiredo Neto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/12301-1 - Synthesis of ordered mesoporous silica with different structures and morphologies
• Grantee: Pedro Leonidas Oseliero Filho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/05888-3 - Biosensors based on amphiphilic peptides for detection and diagnosis of diseases
• Grantee: Barbara Bianca Gerbelli
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/50983-3 - INCT 2014: complex fluids
• Grantee: Antonio Martins Figueiredo Neto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/16092-5 - Investigating colloidal systems by scattering methods
• Grantee: Cristiano Luis Pinto de Oliveira
• Support Opportunities: Regular Research Grants