Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse

Author(s):Show less -

Contato, Alex Graca ^[1] ; de Oliveira, Tassio Brito ^[2] ; Aranha, Guilherme Mauro ^[2] ; de Freitas, Emanuelle Neiverth ^[1] ; Vici, Ana Claudia ^[2] ; Vieira Nogueira, Karoline Maria ^[1] ; de Lucas, Rosymar Coutinho ^{[1, 2]} ; de Almeida Scarcella, Ana Silvia ^[1] ; Buckeridge, Marcos Silveira ^[3] ; Silva, Roberto Nascimento ^[1] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049900 Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14050901 Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo - Brazil

• Document type: Journal article
• Source: MICROORGANISMS; v. 9, n. 3 MAR 2021.
• Web of Science Citations: 0

Abstract

The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. alpha-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-alpha-L-arabinanase, beta-D-galactosidase, beta-D-glucosidase, beta-glucanase, beta-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-beta-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors. (AU)

• FAPESP's process: 18/07522-6 - Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry
• Grantee: Maria de Lourdes Teixeira de Moraes Polizeli
• Support type: Regular Research Grants
• FAPESP's process: 18/25898-3 - Metabolic and genetic engineering of a Saccharomyces cerevisiae industrial strain for transport and co-fermentation of xylose and cellobiose to inprove the ethanol production process from sugarcane bagasse
• Grantee: Karoline Maria Vieira Nogueira
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 17/23989-9 - Lignocellulosic biomass of Panicum maximum Jacq. forage grass produced in simulated future climate conditions: potential source of fermentable sugars for bioenergy
• Grantee: Emanuelle Neiverth de Freitas
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/09000-4 - Effects of temperature and water stress, in climate change scenario, on the fungal community in tropical soil and selection of nitrous oxide producing strains
• Grantee: Tássio Brito de Oliveira
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 17/25862-6 - Structural studies and functional properties of xyloglucanases and lichenases: applications on hydrolysis of lignocellulosic biomass
• Grantee: Alex Graça Contato
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 08/57908-6 - National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants