An Adapted Chronic Constriction Injury of the Sciatic Nerve Produces Sensory, Affective, and Cognitive Impairments: A Peripheral Mononeuropathy Model for the Study of Comorbid Neuropsychiatric Disorders Associated with Neuropathic Pain in Rats

Author(s)

Medeiros, Priscila ^{[1, 2, 3, 4]} ; dos Santos, Ieda Regina ^[4] ; Matias Junior, Ivair ^[5] ; Palazzo, Enza ^[6] ; da Silva, Jose Aparecido ^{[4, 7, 8]} ; Machado, Helio Rubens ^{[2, 3, 5]} ; Ferreira, Sergio Henrique ^{[9, 2, 3]} ; Maione, Sabatino ^{[6, 10]} ; Coimbra, Norberto Cysne ^{[1, 2, 3, 4]} ; de Freitas, Renato Leonardo ^{[9, 2, 3, 4, 7]}

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo FMRP USP, Lab Neurosci Pain & Emot, Ribeirao Preto Med Sch, Dept Surg & Anat, Av Bandeirantes 3900, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo FMRP USP, Neuroelectrophysiol Multiuser Ctr, Ribeirao Preto Med Sch, Dept Surg & Anat, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo FMRP USP, Ribeirao Preto Med Sch, Lab Neuroanat & Neuropsychobiol, Dept Pharmacol, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Lab Pediat Surg, Dept Surg & Anat, Ribeirao Preto, SP - Brazil

^[6] Univ Campania L Vanvitelli, Dept Expt Med, Div Pharmacol, Naples - Italy

^[7] Univ Sao Paulo, Ribeirao Preto Sch Philosophy Sci & Literature, Dept Psychol, Ribeirao Preto, SP - Brazil

^[8] Fed Univ Juiz de Fora UFJF MG, Psychol Dept, Juiz De Fora, MG - Brazil

^[9] Fed Univ Alfenas UNIFAL MG, Biomed Sci Inst, Str Gabriel Monteiro da Silva, Alfenas, MG - Brazil

^[10] IRCCS Neuromed, I-86077 Pozzilli Caserta - Italy

• Document type: Journal article
• Source: PAIN MEDICINE; v. 22, n. 2, p. 338-351, FEB 2021.
• Web of Science Citations: 0

Abstract

Background. Chronic constriction injury (CCI) is a model of neuropathic pain induced by four loose ligatures around the sciatic nerve. This work aimed to investigate the sensory, affective, cognitive, and motor changes induced by an adaptation of the CCI model by applying a single ligature around the sciatic nerve. Methods. Mechanical allodynia was measured from day 1 to day 28 postsurgery by the von Frey test. The beam walking test (BWT) was conducted weekly until 28 days after surgery. Anxiety- and depression-like behaviors, and cognitive performance were assessed through the open field (OF), forced swimming (FS), and novel object recognition (NOR) tests, respectively, 21 days after surgery. Results. The two CCI models, both Bennett and Xie's model (four ligatures of the sciatic nerve) and a modification of it (one ligature), induced mechanical allodynia, increased immobility in the FS, and reduced recognition index in the NOR. The exploratory behavior and time spent in the central part of the arena decreased, while the defensive behavior increased in the OF. The animals subjected to the two CCI models showed motor alterations in the BWT; however, autotomy was observed only in the group with four ligatures and not in the group with a single ligature. Conclusions. Overall these results demonstrate that our adapted CCI model, using a single ligature around the sciatic nerve, induces sensory, affective, cognitive, and motor alterations comparable to the CCI model with four ligatures without generating autotomy. This adaptation to the CCI model may therefore represent an appropriate and more easily performed model for inducing neuropathic pain and study underlying mechanisms and effective treatments. (AU)

• FAPESP's process: 11/19670-0 - Mechanisms involved in the pathophysiology of rheumatoid arthritis, pain and sepsis
• Grantee: Fernando de Queiroz Cunha
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
• Grantee: Fernando de Queiroz Cunha
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/12916-0 - Role of endocannabinoid, glutamatergic and endovanilloid systems of medial prefrontal cortex in neuropathic pain model and investigation of neurological disorders and chronic pain comorbidity
• Grantee: Renato Leonardo de Freitas
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/13560-5 - Effect of deep brain stimulation and cannabidiol treatment in the subthalamic nucleus on the motors and non-Motors symptoms in the experimental model of Parkinson Disease
• Grantee: Priscila Medeiros de Freitas
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/11869-0 - Multi-use equipment approved in grant 2013/12916-0: deep brain stimulation (DBS) (Thomas mini matrix system - Thomas recording GmbH® - Winchester Strasse - Giessen - Germany)
• Grantee: Renato Leonardo de Freitas
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 12/25167-2 - Role of glutamatergic, endocannabinoid and endovaniloid systems of medial pre-frontal cortex in neurophatic pain model: Investigation of panic and chronic pain comorbidity
• Grantee: Priscila Medeiros de Freitas
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/07902-2 - Role of endocannabinoid, glutamatergic and endovanilloid systems of medial prefrontal cortex in neuropathic pain model and investigation of neurological disorders and chronic pain comorbidity
• Grantee: Renato Leonardo de Freitas
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 09/17258-5 - Study of the involvement of nitrergic system and of glutamatergic and cannabinoid-mediated neurotransmission from the medial prefrontal cortex in the analgesia induced by elaborated escape reactions evoked by GABAergic blockade in the medial hypothalamus
• Grantee: Renato Leonardo de Freitas
• Support Opportunities: Scholarships in Brazil - Post-Doctoral