Comparison of foliar spray and soil irrigation of biogenic CuO nanoparticles (NPs) on elemental uptake and accumulation in lettuce

Author(s):Show less -

Kohatsu, Marcio Yukihiro ^[1] ; Pelegrino, Milena Trevisan ^[2] ; Monteiro, Lucilena Rebelo ^[3] ; Freire, Bruna Moreira ^[2] ; Pereira, Rodrigo Mendes ^[2] ; Fincheira, Paola ^[4] ; Rubilar, Olga ^[4] ; Tortella, Gonzalo ^[4] ; Batista, Bruno Lemos ^[2] ; de Jesus, Tatiane Araujo ^[1] ; Seabra, Amedea Barozzi ^[2] ; Lange, Camila Neves ^[2]

Affiliation

^[1] Univ Fed ABC UFABC, Programa Posgrad Ciencia & Tecnol Ambiental CTA, Ave Estados, 5001 Bairro Santa Terezinha, BR-09210580 Santo Andre, SP - Brazil

^[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas, Ave Estados, 5001 Bairro Santa Terezinha, BR-09210580 Santo Andre, SP - Brazil

^[3] Comissao Nacl Energia Nucl, Ipen CNEN SP Inst Pesquisas Energet & Nucl, Ctr Quim & Meio Ambiente, Sao Paulo, SP - Brazil

^[4] Univ La Frontera, Dept Chem Engn, Biotechnol Res Ctr Appl Environm CIBAMA BIOREN, Temuco - Chile

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. 28, n. 13, p. 16350-16367, APR 2021.
• Web of Science Citations: 2

Abstract

Nanoparticles (NPs) can be used in several ways in agriculture, including increasing production rates and improving nutritional values in plants. The present study aims to clarify how biogenic copper oxide nanoparticles (CuO NPs) applied by two routes of exposure (foliar spray and soil irrigation) affect the elemental uptake by lettuce. In vivo experiments using lettuce (n = 4) were performed with CuO NPs in comparison with copper salt (CuSO4), considering a final mass added of 20 mg of CuO per plant. The elemental composition of roots was mostly affected by the soil irrigation exposure for both Cu forms (NPs and salt). Neither Cu form added by soil irrigation was translocated to leaves. Copper concentration in leaves was mainly affected by foliar spray exposure for both Cu forms (NPs and salt). All Cu forms through foliar spray were sequestered in the leaves and no translocation to roots was observed. Foliar spray of CuO NPs caused no visual damage in leaves, resulted in less disturbance of elemental composition, and improved dry weight, number of leaves, CO2 assimilation, and the levels of K, Na, S, Ag, Cd, Cr, Cu, and Zn in leaves without causing significant changes in daily intake of most elements, except for Cu. Although Cu concentration increased in leaves by foliar spray of CuO NPs, it remained safe for consumption. (AU)

• FAPESP's process: 17/05029-8 - Preparation, characterization and biological evaluation of nitric oxide releasing polymeric biomaterials
• Grantee: Milena Trevisan Pelegrino
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/26337-8 - Effects of copper oxide nanoparticles using in vitro and in vivo bioassays with lettuce (Lactuca sativa L.)
• Grantee: Marcio Yukihiro Kohatsu
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/10060-9 - Multi-user equipment (MUE) approved in Grant FAPESP project 2014/05151-0: inductively coupled plasma mass spectrometer hyphenated to an high performance liquid chromatography (HPLC-ICP-MS)
• Grantee: Bruno Lemos Batista
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 17/20914-8 - Method validation for determination of trace elements in urine for occupational evaluation of foundry workers
• Grantee: Bruna Moreira Freire
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants