Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications

Author(s)

Tramontina, Robson ^{[1, 2]} ; Brenelli, Livia Beatriz ^[3] ; Sodre, Victoria ^{[4, 5]} ; Franco Cairo, Joao Paulo ^[4] ; Travalia, Beatriz Medeiros ^[2] ; Egawa, Viviane Yoshimi ^[6] ; Goldbeck, Rosana ^[2] ; Squina, Fabio Marcio ^[4]

Affiliation

^[1] Univ Estadual Campinas UNICAMP, Programa Posgrad Biociencias & Tecnol Prod Bioati, Campinas, SP - Brazil

^[2] State Univ Campinas UNICAMP, Sch Food Engn, Campinas, SP - Brazil

^[3] State Univ Campinas UNICAMP, Interdisciplinary Ctr Energy Planning NIPE, Campinas, SP - Brazil

^[4] Univ Sorocaba UNISO, Programa Proc Tecnol & Ambientais, Sorocaba, SP - Brazil

^[5] Univ Estadual Campinas UNICAMP, Programa Posgrad Biol Func & Mol BFM, Campinas, SP - Brazil

^[6] Sao Paulo State Univ UNESP, Sch Agr, Botucatu, SP - Brazil

• Document type: Review article
• Source: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 36, n. 11 OCT 1 2020.
• Web of Science Citations: 3

Abstract

The physicochemical pretreatment is an important step to reduce biomass recalcitrance and facilitate further processing of plant lignocellulose into bioproducts. This process results in soluble and insoluble biomass fractions, and both may contain by-products that inhibit enzymatic biocatalysts and microbial fermentation. These fermentation inhibitory compounds (ICs) are produced during the degradation of lignin and sugars, resulting in phenolic and furanic compounds, and carboxylic acids. Therefore, detoxification steps may be required to improve lignocellulose conversion by microoganisms. Several physical and chemical methods, such as neutralization, use of activated charcoal and organic solvents, have been developed and recommended for removal of ICs. However, biological processes, especially enzyme-based, have been shown to efficiently remove ICs with the advantage of minimizing environmental issues since they are biogenic catalysts and used in low quantities. This review focuses on describing several enzymatic approaches to promote detoxification of lignocellulosic hydrolysates and improve the performance of microbial fermentation for the generation of bioproducts. Novel strategies using classical carbohydrate active enzymes (CAZymes), such as laccases (AA1) and peroxidases (AA2), as well as more advanced strategies using prooxidant, antioxidant and detoxification enzymes (dubbed as PADs), i.e. superoxide dismutases, are discussed as perspectives in the field. (AU)

• FAPESP's process: 20/03051-9 - Bioprocesses intensification, expansion of chemical products production and lignocellulose processing
• Grantee: Viviane Yoshimi Egawa
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
• Grantee: Fábio Márcio Squina
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 16/07926-4 - Novel integrative strategies through the aldoketo reductase from the termite Coptotermes gestroi for detoxification, saccharification and fermentation of lignocellulose aiming bioethanol production
• Grantee: Robson Tramontina
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/15477-8 - Development of a low severity pretreatment process for the production of sugarcane straw oligosaccharides
• Grantee: Lívia Beatriz Brenelli de Paiva
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 18/18101-1 - Development of the CRISPR/Cas9 system in Rhodococcus jostii RHA1 for production of high-value chemicals from lignin streams
• Grantee: Victoria Ramos Sodré de Castro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)