Diagnostic accuracy of somatic and excretory-secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis

Author(s)

Roldan Gonzales, William Henry ^[1] ; Meisel, Dirce Mary Correia Lima ^[1] ; de Paula, Fabiana Martins ^{[2, 1]} ; Gryschek, Ronaldo Cesar Borges ^{[2, 1]}

Affiliation

^[1] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Imunopatol Esquistossomose, Lab Invest Med, LIM 06, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Med Trop, Fac Med, Sao Paulo - Brazil

• Document type: Journal article
• Source: Parasitology; v. 148, n. 12, p. 1522-1527, OCT 2021.
• Web of Science Citations: 0

Abstract

To evaluate the diagnostic accuracy of three types of antigenic preparations from Strongyloides venezuelensis infective larvae for detection of serum IgG anti-Strongyloides antibodies by enzyme-linked immunosorbent assay (ELISA). Soluble somatic fractions (SSF) and membrane somatic fractions (MSF) and excretory-secretory (E/S) products from S. venezuelensis infective larvae were evaluated against 71 sera from individuals with strongyloidiasis, 105 sera from healthy individuals, and 84 sera from individuals with other helminth infections. Using an ELISA cut-off for 100% sensitivity, E/S products were 97.88% specific followed by MSF (93.12%) and then by SSF (85.2%). The occurrence of cross-reactivity with other helminths was 4.76% (4/84) with E/S products, 8.33% (7/84) with MSF, and 17.86% (15/84) with SSF. For a cut-off for 100% specificity, E/S products showed a sensitivity of 88.73% whereas MSF and SSF showed sensitivities of 59.15% and 53.52%, respectively. In conclusion, E/S products were the best antigenic option for the serodiagnosis of human strongyloidiasis. (AU)

• FAPESP's process: 13/04236-9 - Immunoproteomics applied to the diagnosis human strongyloidiasis used antigen Strongyloides venezuelensis
• Grantee: Ronaldo Cesar Borges Cryschek
• Support Opportunities: Regular Research Grants