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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of a multi-species SNP array for serrasalmid fish Colossoma macropomum and Piaractus mesopotamicus

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Author(s):
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Mastrochirico-Filho, Vito A. [1] ; Ariede, Raquel B. [1] ; Freitas, V, Milena ; Borges, Carolina H. S. [2] ; Lira, Lieschen V. G. [2] ; Mendes, Natalia J. [2] ; Agudelo, John F. G. [2] ; Caceres, Pablo [3] ; Berrocal, Milthon H. M. [4] ; Sucerquia, Gustavo A. L. [5] ; Porto-Foresti, Fabio [2] ; Yanez, Jose M. [3] ; Hashimoto, Diogo T. [2]
Total Authors: 13
Affiliation:
[1] Sao Paulo State Univ, UNESP, Aquaculture Ctr, BR-14884900 Jaboticabal, SP - Brazil
[2] Freitas, Milena, V, Sao Paulo State Univ, UNESP, Aquaculture Ctr, BR-14884900 Jaboticabal, SP - Brazil
[3] Univ Chile, Fac Ciencias Vet & Pecuarias, Santiago - Chile
[4] Univ Nacl Agr Selva, Fac Zootecnia, Tingo Maria - Peru
[5] Univ Antioquia, Fac Ciencias Agr, Medellin - Colombia
Total Affiliations: 5
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 11, n. 1 SEP 29 2021.
Web of Science Citations: 1
Abstract

Scarce genomic resources have limited the development of breeding programs for serrasalmid fish Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu), the key native freshwater fish species produced in South America. The main objectives of this study were to design a dense SNP array for this fish group and to validate its performance on farmed populations from several locations in South America. Using multiple approaches based on different populations of tambaqui and pacu, a final list of 29,575 and 29,612 putative SNPs was selected, respectively, to print an Axiom AFFYMETRIX (THERMOFISHER) SerraSNP array. After validation, 74.17% (n = 21,963) and 71.25% (n = 21,072) of SNPs were classified as polymorphic variants in pacu and tambaqui, respectively. Most of the SNPs segregated within each population ranging from 14,199 to 19,856 in pacu; and from 15,075 to 20,380 in tambaqui. Our results indicate high levels of genetic diversity and clustered samples according to their hatchery origin. The developed SerraSNP array represents a valuable genomic tool approaching in-depth genetic studies for these species. (AU)

FAPESP's process: 16/21011-9 - Integrated analysis of quantitative genetics and genomics to select genotypes of pacu (Piaractus mesopotamicus)
Grantee:Diogo Teruo Hashimoto
Support Opportunities: Regular Research Grants
FAPESP's process: 17/26900-9 - RNA-seq and RAD-seq techniques for genetic characterization of pacu (Piaractus mesopotamicus) resistant to the bacteria Aeromonas hydrophila
Grantee:Vito Antonio Mastrochirico Filho
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 20/11049-4 - Genetic basis for resistance to Aeromonas hydrophila in Pacu (Piaractus mesopotamicus) populations
Grantee:Vito Antonio Mastrochirico Filho
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/18294-9 - Integration of genomic methods for genotypes selection of pacu (Piaractus mesopotamicus) resistant to Aeromonas hydrophila bacteria
Grantee:Vito Antonio Mastrochirico Filho
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 18/08416-5 - Low-cost genome-wide association study and genomic predictions for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)
Grantee:Diogo Teruo Hashimoto
Support Opportunities: Regular Research Grants
FAPESP's process: 17/19717-3 - Genome analysis for SNPs association with resistance to Aeromonas hydrophila and fillet yield in tambaqui (Colossoma macropomum)
Grantee:Raquel Belini Ariede
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 19/10662-7 - Genome wide association study to resistance against Aeromonas hydrophila in tambaqui (Colossoma macropomum)
Grantee:Raquel Belini Ariede
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 19/08972-8 - Evaluation of the genetic diversity in Colossoma macropomum for pre-breeding programs in Brazil and Peru
Grantee:Diogo Teruo Hashimoto
Support Opportunities: Regular Research Grants