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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spermatozoa and seminal plasma small extracellular vesicles miRNAs as biomarkers of boar semen cryotolerance

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Author(s):
Pedrosa, Ana Carolina [1] ; Torres, Mariana Andrade [1] ; Alkmin, Diego Vilela [2] ; Pinzon, Jorge E. P. [3] ; Massami Kitamura Martins, Simone Maria [4] ; da Silveira, Juliano Coelho [3] ; Cesar de Andrade, Andre Furugen [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Pirassununga, SP - Brazil
[2] DB Swine Genet, Patos De Minas, MG - Brazil
[3] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP - Brazil
[4] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Theriogenology; v. 174, p. 60-72, OCT 15 2021.
Web of Science Citations: 0
Abstract

Freeze boar semen is still the biggest challenge for the swine industry due to the high cold shock sensitivity of boar sperm cells and the variance of post-thaw results among individuals and ejaculates from the same boar. To solve this problem, we investigate if miRNAs present in sperm cells and small extracellular vesicles (EVs) from seminal plasma of raw boar ejaculates can predict high-quality ejaculates after underwent the freeze-thaw process. For this, we obtained miRNAs samples of sperm cells and EVs from raw seminal plasma from 27 ejaculates before the cryopreservation process. Two groups with different freezability considering the analysis post-thaw of structure and sperm functionality were formed: High freezability (HF; n = 04) and low freezability (LF; n = 04). That done, we investigated the miRNAs profile of sperm cells and EVs from seminal plasma in both groups. Three miRNAs were differently abundant in LF ejaculates, being the ssc-miR-503 found in higher levels in sperm cells (P < 0.10). The ssc-miR-130a and ssc-miR-9 most abundant in EVs from seminal plasma (P < 0.10), in LF ejaculates. Through enrichment analysis, it was possible to verify that these miRNAs could be performing modifications in the development of male germ cells and in the production of energy to spermatozoa to maintain their viability and functionality. Therefore, we can demonstrate that ssc-miR-503, ssc-miR130a, and ssc-miR-9 are related to low sperm cryotolerance in boars semen. So those miRNAs can be used as a biomarker to predict their low ability to tolerate the cryopreservation process. (c) 2021 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 16/24690-4 - Metabolomics signatures on the influence of holding time on increasing boar spermatozoa cryotolerance
Grantee:André Furugen Cesar de Andrade
Support Opportunities: Regular Research Grants
FAPESP's process: 18/12500-1 - Study of the miRNAs profile of boars ejaculates with different freezability
Grantee:Ana Carolina Pedrosa
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/21829-9 - Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10
Grantee:Juliano Coelho da Silveira
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support Opportunities: Research Grants - Young Investigators Grants