| Full text | |
| Author(s): |
Ataide, Vanessa N.
[1]
;
Rocha, Diego P.
[1]
;
de Siervo, Abner
[2]
;
Paixao, Thiago R. L. C.
[1]
;
Munoz, Rodrigo A. A.
[3]
;
Angnes, Lucio
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Dept Appl Phys, BR-13083859 Campinas, SP - Brazil
[3] Univ Fed Uberlandia, Inst Chem, BR-38400902 Uberlandia, MG - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Microchimica Acta; v. 188, n. 11 NOV 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
For the first time the development of an electrochemical method for simultaneous quantification of Zn2+ and uric acid (UA) in sweat is described using an electrochemically treated 3D-printed working electrode. Sweat analysis can provide important information about metabolites that are valuable indicators of biological processes. Improved performance of the 3D-printed electrode was achieved after electrochemical treatment of its surface in an alkaline medium. This treatment promotes the PLA removal (insulating layer) and exposes carbon black (CB) conductive sites. The pH and the square-wave anodic stripping voltammetry technique were carefully adjusted to optimize the method. The peaks for Zn2+ and UA were well-defined at around - 1.1 V and + 0.45 V (vs. CB/PLA pseudo-reference), respectively, using the treated surface under optimized conditions. The calibration curve showed a linear range of 1 to 70 mu g L-1 and 1 to 70 mu mol L-1 for Zn2+ and UA, respectively. Relative standard deviation values were estimated as 4.8% (n = 10, 30 mu g L-1) and 6.1% (n = 10, 30 mu mol L-1) for Zn2+ and UA, respectively. The detection limits for Zn2+ and UA were 0.10 mu g L-1 and 0.28 mu mol L-1, respectively. Both species were determined simultaneously in real sweat samples, and the achieved recovery percentages were between 95 and 106% for Zn2+ and 82 and 108% for UA. (AU) | |
| FAPESP's process: | 18/14462-0 - Fabrication of electrochemical paper-based device aiming clinical and environmental applications |
| Grantee: | Vanessa Neiva de Ataide |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 20/00325-0 - Production of conductive filaments containing carbon nanomaterials and metal nanoparticles for the development of low cost enzymeless electrochemical biosensors via additive manufacturing |
| Grantee: | Diego Pessoa Rocha |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 07/08244-5 - Study of the magnetic properties of Pd and PdAu ultrathin films and nanoparticles by using XMCD |
| Grantee: | Abner de Siervo |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 07/54829-5 - Electronic and geometric structure of nano-materials: synchrotron radiation studies |
| Grantee: | Richard Landers |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Research Projects - Thematic Grants |