| Full text | |
| Author(s): |
de Assis, Leonardo Vinicius Monteiro
[1, 2]
;
Moraes, Maria Nathalia
[1, 3]
;
Mendes, Davi
[4]
;
Silva, Matheus Molina
[4]
;
Menck, Carlos Frederico Martins
[4]
;
Castrucci, Ana Maria de Lauro
[5, 1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Lab Comparat Physiol Pigmentat, Dept Physiol, Inst Biosci, BR-05508090 Sao Paulo - Brazil
[2] Univ Lubeck, Inst Neurobiol, Ctr Brain Behav & Metab, D-23562 Lubeck - Germany
[3] Univ Sao Paulo, Neurobiol Lab, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508000 Sao Paulo - Brazil
[4] Univ Sao Paulo, DNA Repair Lab, Dept Microbiol, Inst Biomed Sci, BR-05508000 Sao Paulo - Brazil
[5] Univ Virginia, Dept Biol, Charlottesville, VA 22904 - USA
Total Affiliations: 5
|
| Document type: | Review article |
| Source: | CURRENT ISSUES IN MOLECULAR BIOLOGY; v. 43, n. 3, p. 1436-1450, DEC 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Skin melanocytes harbor a complex photosensitive system comprised of opsins, which were shown, in recent years, to display light- and thermo-independent functions. Based on this premise, we investigated whether melanopsin, OPN4, displays such a role in normal melanocytes. In this study, we found that murine Opn4(KO) melanocytes displayed a faster proliferation rate compared to Opn4(WT) melanocytes. Cell cycle population analysis demonstrated that OPN4(KO) melanocytes exhibited a faster cell cycle progression with reduced G(0)-G(1), and highly increased S and slightly increased G(2)/M cell populations compared to the Opn4(WT) counterparts. Expression of specific cell cycle-related genes in Opn4(KO) melanocytes exhibited alterations that corroborate a faster cell cycle progression. We also found significant modification in gene and protein expression levels of important regulators of melanocyte physiology. PER1 protein level was higher while BMAL1 and REV-ERB alpha decreased in Opn4(KO) melanocytes compared to Opn4(WT) cells. Interestingly, the gene expression of microphthalmia-associated transcription factor (MITF) was upregulated in Opn4(KO) melanocytes, which is in line with a higher proliferative capability. Taken altogether, we demonstrated that OPN4 regulates cell proliferation, cell cycle, and affects the expression of several important factors of the melanocyte physiology; thus, arguing for a putative tumor suppression role in melanocytes. (AU) | |
| FAPESP's process: | 18/16511-8 - Involvement of the photosensitive and temporal controlling systems in the development, progression, and metastasis of malignant melanoma: an investigation of novel therapeutic targets |
| Grantee: | Leonardo Vinícius Monteiro de Assis |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/24615-5 - Breaking a paradigm? Melanopsin, a canonical photo-pigment, acting as sensor to entrain the clock in light unexposed organs, and its putative interaction with TRP channels: a trans-disciplinary study involving physiological and pathological aspects |
| Grantee: | Ana Maria de Lauro Castrucci |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/26651-9 - Glaucoma as a model of temporal information disruption: impact on the metabolism |
| Grantee: | Maria Nathália de Carvalho Magalhães Moraes Figueira Borges |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 17/18781-0 - The role of XPG endonuclease in nuclear RNA transcription and in the maintenance of mitochondrial genome |
| Grantee: | Davi Mendes |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 19/19435-3 - The role of DNA damage and mitochondrial function in vascular, immune and neurological ageing (DNA MoVINg) |
| Grantee: | Carlos Frederico Martins Menck |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/14728-0 - Melanopsin as the UVA photoreceptor and its relationship with pigmentation, DNA repair, biological clock and components of the HPA axis: a novel pharmacological target? |
| Grantee: | Ana Maria de Lauro Castrucci |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/24217-0 - Mechanisms of tumor resistance to cisplatin: DNA lesions processing and circadian cycle effect |
| Grantee: | Matheus Molina Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |