ALDI-TOF mass spectrometry of saliva samples as a prognostic tool for COVID-1

Author(s):Show less -

Lazari, Lucas C. ^[1] ; Zerbinati, Rodrigo M. ^[2] ; Rosa-Fernandes, Livia ^{[1, 3]} ; Santiago, Veronica Feijoli ^[1] ; Rosa, Klaise F. ^[1] ; Angeli, Claudia B. ^[1] ; Schwab, Gabriela ^[2] ; Palmieri, Michelle ^[4] ; Sarmento, Dmity J. S. ^[4] ; Marinho, Claudio R. F. ^[3] ; Almeida, Janete Dias ^[5] ; To, Kelvin ^[6] ; Giannecchini, Simone ^[7] ; Wrenger, Carsten ^[8] ; Sabino, Ester C. ^[9] ; Martinho, Herculano ^[10] ; Lindoso, Jose A. L. ^{[11, 12, 13]} ; Durigon, Edison L. ^[14] ; Braz-Silva, Paulo H. ^{[4, 2]} ; Palmisano, Giuseppe ^[1]

Affiliation:Show less -

^[1] Univ Sao Paulo, Dept Parasitol, GlycoProte Lab, ICB, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Inst Trop Med Sao Paulo, Lab Virol LIM HC FMUSP 52, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Parasitol, Lab Expt Immunoparasitol, ICB, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil

^[5] Sao Paulo State Univ, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos - Brazil

^[6] Univ Hong Kong, Carol Yu Ctr Infect, Dept Microbiol, State Key Lab Emerging Infect Dis, Li KaShing Fac, Hong Kong - Peoples R China

^[7] Univ Florence, Dept Expt & Clin Med, Florence - Italy

^[8] Univ Sao Paulo, Dept Parasitol, Unit Drug Discovery, ICB, Sao Paulo - Brazil

^[9] Univ Sao Paulo, Sch Med, Inst Trop Med Sao Paulo, Sao Paulo - Brazil

^[10] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil

^[11] Univ Sao Paulo, Sch Med, Dept Infect Dis, Sao Paulo - Brazil

^[12] Inst Infect Dis Emilio Ribas, Sao Paulo - Brazil

^[13] Univ Sao Paulo, Sch Med, Inst Trop Med Sao Paulo, Lab Protozool LIM HC FMUSP 49, Sao Paulo - Brazil

^[14] Univ Sao Paulo, Dept Microbiol, Lab Clin & Mol Virol, ICB, Sao Paulo - Brazil

• Document type: Journal article
• Source: OURNAL OF ORAL MICROBIOLOG; v. 14, n. 1 DEC 31 2022.
• Web of Science Citations: 0

Abstract

Background The SARS-CoV-2 infections are still imposing a great public health challenge despite the recent developments in vaccines and therapy. Searching for diagnostic and prognostic methods that are fast, low-cost and accurate are essential for disease control and patient recovery. The MALDI-TOF mass spectrometry technique is rapid, low cost and accurate when compared to other MS methods, thus its use is already reported in the literature for various applications, including microorganism identification, diagnosis and prognosis of diseases. Methods Here we developed a prognostic method for COVID-19 using the proteomic profile of saliva samples submitted to MALDI-TOF and machine learning algorithms to train models for COVID-19 severity assessment. Results We achieved an accuracy of 88.5%, specificity of 85% and sensitivity of 91.5% for classification between mild/moderate and severe conditions. When we tested the model performance in an independent dataset, we achieved an accuracy, sensitivity and specificity of 67.18, 52.17 and 75.60% respectively. Conclusion Saliva is already reported to have high inter-sample variation; however, our results demonstrates that this approach has the potential to be a prognostic method for COVID-19. Additionally, the technology used is already available in several clinics, facilitating the implementation of the method. Further investigation using a larger dataset is necessary to consolidate the technique. (AU)

• FAPESP's process: 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications
• Grantee: Giuseppe Palmisano
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 20/06409-1 - Evaluation of humoral immune response and inflammatory response in patients with confirmed diagnosis of COVID-19 at Hospital Sírio Libanês and correlation with disease severity
• Grantee: Edison Luiz Durigon
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/04923-0 - SARS-CoV-2 glycosylation: a blueprint structural insight for understanding COVID-19 pathogenesis
• Grantee: Giuseppe Palmisano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins
• Grantee: Giuseppe Palmisano
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 15/26722-8 - Drug discovery against human infectious diseases
• Grantee: Carsten Wrenger
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/03966-4 - Targeting lipoic acid salvage and biosynthesis pathways in MRSA
• Grantee: Carsten Wrenger
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/07490-0 - Salivary biomarkers of COVID-19 and other viral respiratory infections
• Grantee: Paulo Henrique Braz da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/20468-0 - Recrudescence of the malaria during pregnancy: effects and mechanisms
• Grantee: Cláudio Romero Farias Marinho
• Support Opportunities: Regular Research Grants