Fractionation of sesquiterpenes and diterpenic acids from copaiba (Copaifera officinalis) oleoresin using supercritical adsorption

Author(s)

Luis Pasquel-Reategui, Jose ; dos Santos, Luana Cristina ; Barrales, Francisco Manuel ; Grober, Vanessa Lais ; Soares Forte, Marcus Bruno ; Sartoratto, Adilson ; Queiroga, Carmen Lucia ; Martinez, Julian

• Document type: Journal article
• Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 184, p. 10-pg., 2022-05-01.

Abstract

Copaifera officinalis (known as 'copaiba') has shown potential application in food and pharmaceutical industries, where biological activities of terpenes are highlighted in the literature. However, clean technologies to fractionate these value-added compounds are still unexplored in literature. This work aimed to separate terpenes from copaiba oleoresin with supercritical CO2 adsorption (SFA), using three adsorbents (zeolite 13X, aluminum oxide, and silicon oxide) at pressures of 9-29 MPa and temperature of 50 ?. At 9 MPa, zeolite 13X retained beta-caryophyllene, achieving concentration factors (CF beta-C) below 0.48, despite good purification efficacy, with the purification factor (PF beta-C) = 1.25. Aluminum and silicon oxides were selective to beta-caryophyllene, achieving CF beta-C > 1. Therefore, a two-step adsorption-desorption process could obtain highly concentrated beta-caryophyllene fractions. Copalic acid remained adsorbed at 9 MPa in SFA with zeolite 13X. At this condition, CFCA and PFCA over 2 and 5 were obtained, respectively, after zeolite desorption with ethyl acetate. (AU)

• FAPESP's process: 17/23670-2 - Biorefinery for the recovery of by-products from passion fruit processing through high pressure technologies
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/11932-7 - Integration of high pressure extraction to separation operations for the recovery of by-products from fruit processing
• Grantee: Julian Martínez
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/13602-7 - Study on strategies to allow the integral use of lignocellulosic biomass
• Grantee: Camila Alves de Rezende
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 17/11245-5 - Fractionation of passion fruit (Passiflora edulis) by-products extracts by reducing pressure using supercritical CO2
• Grantee: Luana Cristina dos Santos
• Support Opportunities: Scholarships in Brazil - Doctorate