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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of insect-specific Culex flavivirus (Flaviviridae) nucleotide sequences in mosquitoes from urban parks in São Paulo, Brazil

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Author(s):
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Thaís de Moura Coletti ; Camila Malta Romano [2] ; Paulo Roberto Urbinatti [3] ; Renildo Souza Teixeira [4] ; Leila Weiss de Almeida Pedrosa [5] ; Marcello Schiavo Nardi [6] ; Delsio Natal [7] ; Antônio Charlys da Costa [8] ; Jaqueline Goes de Jesus [9] ; Ingra Morales Claro [10] ; Ester Cerdeira Sabino [11] ; Steven S. Witkin ; Mauro Toledo Marrelli [13] ; Licia Natal Fernandes [14]
Total Authors: 14
Document type: Journal article
Source: Revista da Sociedade Brasileira de Medicina Tropical; v. 55, 2022-09-26.
Abstract

ABSTRACT Background: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. Methods: We evaluated the presence of ISFs in mosquitoes collected in São Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. Results: Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). Conclusions: The identification of CxFV genotype 2 at new locations in São Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence. (AU)

FAPESP's process: 16/18204-0 - Flavivirus and Alphavirus identification in mosquitoes (Diptera: Culicidae) collected in green spaces of the city of São Paulo
Grantee:Thaís de Moura Coletti
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 19/03859-9 - Clinical, serological and virological characteristics of Dengue virus serotype 2 (DENV-2) infection in a cohort previously exposed to serotype 1
Grantee:Camila Malta Romano
Support Opportunities: Regular Research Grants
FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
Grantee:Antonio Charlys da Costa
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 19/12000-1 - Modeling of the spatiotemporal dispersion of emergent arboviruses using heterogeneous data sources in the State of São Paulo
Grantee:Jaqueline Goes de Jesus
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 10/51230-8 - Biodiversity of mosquitoes (Diptera : Culicidae) in the city of São Paulo municipal parks
Grantee:Mauro Toledo Marrelli
Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
FAPESP's process: 18/14389-0 - Brazil-UK Centre for Arbovirus Discovery, Diagnosis, Genomics and Epidemiology (CADDE)
Grantee:Ester Cerdeira Sabino
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/17176-8 - Real-time, rapid detection and sequencing of arboviruses in Brazil: Zika, Dengue, Chikungunya and Yellow Fever
Grantee:Ingra Morales Claro
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)