Screening of potential endoglucanases, hydrolysis conditions and different sugarcane straws pretreatments for cello-oligosaccharides production

Author(s)

Barbosa, Fernando Cesar ; Martins, Manoela ; Brenelli, Livia Beatriz ; Ferrari, Felipe Augusto ; Soares Forte, Marcus Bruno ; Rabelo, Sarita Candida ; Franco, Telma Teixeira ; Goldbeck, Rosana

• Document type: Journal article
• Source: Bioresource Technology; v. 316, p. 8-pg., 2020-11-01.

Abstract

Cello-oligosaccharides (COS) are oligomers with 2 to 6 beta-1,4-linked glucose units, with potential applications in the food/feed and bioenergy industrial sectors. In this study, the combination of five heterologous expressed endoglucanases varying the temperature and pH conditions were evaluated by design of experiments for COS production. Afterwards, the best combination was tested to produce COS from different pretreated sugarcane straws: ionic liquid, diluted acid, hydrothermal and steam-explosion. The results showed that steam explosion pretreated sugarcane straw treated with CtCel9R enzyme at 50 degrees C and pH 5.0 yielded 13.4 mg COS g biomass(-1), 5-18-fold higher compared to the other pretreated straws. Under the conditions evaluated, the removal of hemicellulose and decrease in the cellulose crystallinity can benefits the enzymatic hydrolysis. This is the first study that combined the evaluation of different enzymes, conditions, and sugarcane straw pretreatments to optimize COS production in a single step without glucose formation. (AU)

• FAPESP's process: 18/14223-5 - Obtaining symbiotic microcapsules of xylo-oligosaccharides from sugarcane straw
• Grantee: Manoela Martins
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/08542-3 - Enzymatic biotechnology for the xylo-oligosaccharides production from lignocellulosic agroindustrial residues aimed the production of symbiotics
• Grantee: Rosana Goldbeck Coelho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/15477-8 - Development of a low severity pretreatment process for the production of sugarcane straw oligosaccharides
• Grantee: Lívia Beatriz Brenelli de Paiva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
• Grantee: Telma Teixeira Franco
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/19976-4 - Lignocellulosic biomass processing using ionic liquids for biorefinery application
• Grantee: Felipe Augusto Ferrari
• Support Opportunities: Scholarships in Brazil - Doctorate