Blood orange juice intake modulates plasma and PBMC microRNA expression in overweight and insulin-resistant women: impact on MAPK and NF Kappa B signaling pathways

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Capetini, Vinicius Cooper ; Quintanilha, Bruna J. ; de Oliveira, Dalila Cunha ; Nishioka, Alessandra Harumi ; de Matos, Luciene Assaf ; Ferreira, Ludmila Rodrigues Pinto ; Ferreira, Frederico Moraes ; Sampaio, Geni Rodrigues ; Hassimotto, Neuza Mariko Aymoto ; Lajolo, Franco Maria ; Fock, Ricardo Ambrosio ; Rogero, Marcelo Macedo

• Document type: Journal article
• Source: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 112, p. 14-pg., 2022-12-17.

Abstract

Blood orange consumption presents potential health benefits and may modulate epigenetic mechanisms such as microRNAs (miRNAs) expression. MiR-NAs are non-coding RNAs responsible for post-transcriptional gene regulation, and these molecules can also be used as biomarkers in body fluids. This study was designed to investigate the effect of chronic blood orange juice (BOJ) intake on the inflammatory response and miRNA expression profile in plasma and blood cells in overweight women. The study cohort was comprised of twenty women aged 18-40 years old, diagnosed as overweight, who consumed 500 mL/d of BOJ for four weeks. Clinical data were collected at baseline and after 4 weeks of juice consumption, e.g., anthropometric and hemodynamic parameters, food intake, blood cell count, and metabolic and inflammatory biomarkers. BOJ samples were analyzed and characterized. Additionally, plasma and blood cells were also collected for miRNA expression profiling and evaluation of the expression of genes and proteins in the MAPK and NF Kappa B signaling pathways. BOJ intake increased the expression of miR-144-3p in plasma and the expression of miR-424-5p, miR-144-3p, and miR-130b-3p in peripheral blood mononuclear cells (PBMC). Conversely, the beverage intake decreased the expression of let-7f-5p and miR-126-3p in PBMC. Computational analy-ses identified different targets of the dysregulated miRNA on inflammatory pathways. Furthermore, BOJ intake increased vitamin C consumption and the pJNK/JNK ratio and decreased the expression of IL6 mRNA and NF Kappa B protein. These results demonstrate that BOJ regulates the expression of genes involved in the inflammatory process and decreases NF k B-protein expression in PBMC.(c) 2022 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 18/25046-7 - Chronic effect of blood orange juice intake on microRNA expression profile and inflammatory response in overweight women
• Grantee: Vinícius Cooper Capetini
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC