The association of modified QuEChERS and DLLME to offer high analytical detectability to assess residual depletion profile of erythromycin in fish

Author(s)

Campanharo, Sarah Chagas ; da Silva, Agnaldo Fernando Baldo ; Bleuzen, Anais ; da Silva, Jonas Joaquim Mangabeira ; de Freitas, Lucas Victor Pereira ; Assane, Inacio Mateus ; Pilarski, Fabiana ; Paschoal, Jonas Augusto Rizzato

• Document type: Journal article
• Source: Food Chemistry; v. 405, p. 8-pg., 2023-03-30.

Abstract

An analytical method for the determination of erythromycin A (ERY) residues in fish fillet was developed, optimized, and validated employing a modified QuEChERS procedure associated to DLLME technique as a preconcentration step. The obtained LOD and the LOQ were 0.1 mu g kg- 1 and 1 mu g kg- 1, respectively. The validated method provides linearity in the range of 1 to 20 mu g kg- 1, precision (CV < 6.3 %) and accuracy (recovery ranging from 103 to 110 %). The procedure was applied in an experimental study to evaluate the residual depletion profile of ERY in fish (Piaractus mesopotamicus) after oral administration. The treatment was carried out at a daily dose of 100 mg (kg BW)-1 of ERY, for 7 consecutive days and with an average water temperature of 30 <degrees>C. A withdrawal time of 240 degrees -day was estimated for eliminating ERY residues at concentration levels below the maximum residue limit considered (MRL 100 mu g kg- 1). (AU)

• FAPESP's process: 12/18334-0 - Veterinary drug residues in food: multiclass analytical methods employing LC-MS/MS, exposition data and residual depletion study
• Grantee: Jonas Augusto Rizzato Paschoal
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/06159-8 - Studies for the safe use of erythromycin and doxycycline in fish of national commercial importance
• Grantee: Jonas Augusto Rizzato Paschoal
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/15052-7 - Residual persistence investigation of toxic constituents of C. duckei used in Pisciculture
• Grantee: Jonas Joaquim Mangabeira da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/05536-0 - Erythromycin in aquaculture: studies for drug incorporation in feed and for residual depletion in fish
• Grantee: Sarah Chagas Campanharo
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/22775-0 - Combination of thiamphenicol with florfenicol: pharmacokinetics and therapeutic efficacy in the treatment of Streptococcosis in Nile Tilapia (Oreochromis niloticus)
• Grantee: Inácio Mateus Assane
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/03294-1 - Studies for the use of levamisole in Aquaculture: analytical methods; incorporation in the feed; residual depletion; and withdrawal period estimate
• Grantee: Lucas Victor Pereira de Freitas
• Support Opportunities: Scholarships in Brazil - Doctorate