| Full text | |
| Author(s): |
Sua-Cespedes, Cristhian
;
Lacerda, Jose Thalles
;
Zanetti, Giovanna
;
David, Daniela Dantas
;
Moraes, Maria Nathalia
;
de Assis, Leonardo V. M.
;
Castrucci, Ana Maria L.
Total Authors: 7
|
| Document type: | Journal article |
| Source: | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 242, p. 15-pg., 2023-04-03. |
| Abstract | |
The presence of melanopsin (OPN4) has been shown in cultured murine melanocytes and was associated with ultraviolet A radiation (UVA) reception. Here we demonstrated the protective role of OPN4 in skin physiology and the increased UVA-induced damage in its absence. Histological analysis showed a thicker dermis and thinner hypodermal white adipose tissue layer in Opn4-/- (KO) mice than in wild-type (WT) animals. Proteomics analyses revealed molecular signatures associated with proteolysis, remodeling chromatin, DNA damage response (DDR), immune response, and oxidative stress coupled with antioxidant responses in the skin of Opn4 KO mice compared to WT. Skin protein variants were found in Opn4 KO mice and Opn2, Opn3, and Opn5 gene expressions were increased in the genotype. We investigated each genotype response to UVA stimulus (100 kJ/m2). We found an increase of Opn4 gene expression following stimulus on the skin of WT mice suggesting melanopsin as a UVA sensor. Proteomics findings suggest that UVA decreases DDR pathways associated with ROS accumulation and lipid peroxidation in the skin of Opn4 KO mice. Relative changes in methylation (H3-K79) and acetylation sites of histone between genotypes and differentially modulated by UVA stimulus were also observed. We also identified alterations of molecular traits of the central hypothalamus-pituitary- adrenal (HPA) and the skin HPA-like axes in the absence of OPN4. Higher skin corticosterone levels were detected in UVA-stimulated Opn4 KO compared to irradiated WT mice. Taken altogether, functional proteomics associated with gene expression experiments allowed a high-throughput evaluation that suggests an important protective role of OPN4 in regulating skin physiology in the presence and absence of UVA radiation. (AU) | |
| FAPESP's process: | 16/00696-3 - Proteomic as a tool to understand HDL function and composition |
| Grantee: | Graziella Eliza Ronsein |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine |
| Grantee: | Ohara Augusto |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 19/19005-9 - Glaucoma as a model of chronodisruption: impact on the metabolism |
| Grantee: | Maria Nathália de Carvalho Magalhães Moraes Figueira Borges |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |
| FAPESP's process: | 21/01659-2 - Participation of melanopsin in the thermoregulation of mammals |
| Grantee: | Giovanna Zanetti |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 17/26651-9 - Glaucoma as a model of temporal information disruption: impact on the metabolism |
| Grantee: | Maria Nathália de Carvalho Magalhães Moraes Figueira Borges |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 18/23043-0 - Photo- and termo-reception systems in the Blue Crab, Callinectes sapidus (Crustacea Brachyura), and their relationship with the molt cycle |
| Grantee: | Daniela Dantas David |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/16511-8 - Involvement of the photosensitive and temporal controlling systems in the development, progression, and metastasis of malignant melanoma: an investigation of novel therapeutic targets |
| Grantee: | Leonardo Vinícius Monteiro de Assis |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/50214-4 - Biological clock setting by light and temperature: phylogenetic aspects |
| Grantee: | Ana Maria de Lauro Castrucci |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 12/12663-1 - Singlet molecular oxygen and peroxides in chemical biology |
| Grantee: | Paolo Di Mascio |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/14728-0 - Melanopsin as the UVA photoreceptor and its relationship with pigmentation, DNA repair, biological clock and components of the HPA axis: a novel pharmacological target? |
| Grantee: | Ana Maria de Lauro Castrucci |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/24615-5 - Breaking a paradigm? Melanopsin, a canonical photo-pigment, acting as sensor to entrain the clock in light unexposed organs, and its putative interaction with TRP channels: a trans-disciplinary study involving physiological and pathological aspects |
| Grantee: | Ana Maria de Lauro Castrucci |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 20/04524-8 - Integrating proteogenomics approach and system biology to investigate the role of melanopsin and TRP channels in the circadian rhythmicity |
| Grantee: | José Thalles Jocelino Gomes de Lacerda |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/16412-9 - Mechanisms of clock genes thermo-modulation in peripheral tissue of mammals: role of TRP channels |
| Grantee: | Maria Nathália de Carvalho Magalhães Moraes Figueira Borges |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |