Clinical evaluation of culturable bacteria, endotoxins and lipoteichoic acid in teeth with vital normal pulp tissues

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Chiarelli-Neto, Vito M. ; de Aveiro, Emelly ; Bronzato, Juliana D. ; Arruda-Vasconcelos, Rodrigo ; Louzada, Lidiane M. ; Godoi, Ederaldo P. ; Lopes, Erica M. ; de-Jesus-Soares, Adriana ; Ferraz, Caio C. R. ; Almeida, Jose F. A. ; Marciano, Marina A. ; Gomes, Brenda P. F. A.

• Document type: Journal article
• Source: AUSTRALIAN ENDODONTIC JOURNAL; v. N/A, p. 9-pg., 2023-08-01.

Abstract

The objective of this study was to investigate the presence of culturable bacteria, endotoxins (LPS) and lipoteichoic acid (LTA) levels in teeth with normal vital pulp (NVP) with intact crowns (IC) and those with coronal restoration (CR) limited to the enamel level. A total of 20 teeth indicated for endodontic treatment due to prosthetic reasons were selected. Samples were collected from the root canals. The levels of cultivable bacteria, LPS and LTA were assessed. Statistical analyses were performed at significance level set at 5%. None of the teeth presented microbial growth. In the IC group, the LPS levels were limited to the lowest concentration of LPS. On the contrary, higher LPS and LTA levels were detected in teeth with CR. It was concluded that teeth with NVP and IC were negative for bacteria, LPS and LTA; while teeth with CR were positive for bacterial virulence factors. (AU)

• FAPESP's process: 21/13871-6 - Decoding the microbiota correlations of teeth indicated for endodontic retreatment and the complex interrelationship between mobiloma and resistome in oral site metagenomes
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/14448-0 - Microbiomes and immunobiological aspects in endodontic infections
• Grantee: Erica Mendes Lopes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 21/14570-0 - INFLUENCE OF THE INFECTION TIME ON THE MICROBIOLOGICAL (BACTERIA AND FUNGI), CYTOTOXIC AND INFLAMMATORY PROFILE OF THE MICROBIAL COMMUNITY OF SECONDARY/PERSISTENT ENDODONTIC INFECTIONS
• Grantee: Ederaldo Pietrafesa de Godoi Junior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/25242-8 - Clinical, microbiological and immunological monitoring of patients undertaken to endodontic therapy with diagnosis of irreversible Pulpitis
• Grantee: Rodrigo Arruda Vasconcelos
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/25090-3 - Microbiological and fadA family analysis in root canals and periapical lesions of teeth with secondary infection and their relation with the levels of proinflammatory cytokines and MMPs
• Grantee: Juliana Delatorre Bronzato
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/19300-0 - Investigation of the clinical, microbiological and immunological aspects of teeth with diagnosis of primary periodontal lesions with secondary endodontic involvement
• Grantee: Lidiane Mendes Louzada
• Support Opportunities: Scholarships in Brazil - Doctorate