Removal of 5-hydroxymethylfurfural from brewer's spent grains hydrolysates obtained by subcritical water hydrolysis: An approach using liquid-liquid extraction

Author(s)

Barroso, Tiago Linhares Cruz Tabosa ; Sganzerla, William Gustavo ; Castro, Luiz Eduardo Nochi ; Freiria, Nicolas Luis Moreira ; Barbero, Gerardo Fernandez ; Lovillo, Miguel Palma ; Rostagno, Mauricio Ariel ; Forster-Carneiro, Tania

• Document type: Journal article
• Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 201, p. 9-pg., 2023-06-13.

Abstract

This study evaluated the use of a semi-continuous subcritical water hydrolysis (SWH) process followed by liquid -liquid extraction to produce a concentrated hydrolysate with a low content of 5-hydroxymethylfurfural (5-HMF). The SWH was operated at 15 MPa, 180 degrees C, 5 mL min -1, and a solvent-to-feed ratio of 22.5 g g-1. The hydrolysate was subjected to liquid-liquid extraction at different pH (2.5-6.5), temperatures (25-45 degrees C), and volumetric ratios between hydrolysate and ethyl acetate (1:2-2:1 v/v) using the Box-Behnken design followed by response surface methodology. The optimal conditions for removing 5-HMF (73.85 %) were pH 6.5, 35 degrees C, and a volu-metric ratio between hydrolysate and ethyl acetate of 1:2 (v/v). The hydrolysate presented a final composition of 14.82 g L-1 cellobiose, 10.83 g L-1 glucose, 6.36 g L-1 xylose, 3.61 g L-1 arabinose, 2.47 g L-1 formic acid, 3.75 g L-1 acetic acid, and 1.04 g L-1 5-HMF. (AU)

• FAPESP's process: 21/04096-9 - Integration of the extraction/hydrolysis processes in subcritical water and purification for the valuation of grape bagasse in a biorrefinery concept
• Grantee: Luiz Eduardo Nochi de Castro
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 18/14938-4 - Waste2energy: integration of supercritical technologies and anaerobic digestion through an automated system for the production of biofuel precursors
• Grantee: Tânia Forster Carneiro
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 20/16248-5 - Purification of jabuticaba waste extracts by adsorbents and biochar using supercritical technology
• Grantee: Tiago Linhares Cruz Tabosa Barroso
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/26925-7 - Development of an automated system for biofuel precursor production using sub-critical technology
• Grantee: William Gustavo Sganzerla
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)