Advanced search
Start date
Betweenand


Transcriptome profile of skeletal muscle using different sources of dietary fatty acids in male pigs

Full text
Author(s):
Show less -
Fanalli, Simara Larissa ; da Silva, Bruna Pereira Martins ; Gomes, Julia Dezen ; de Almeida, Vivian Vezzoni ; Moreira, Gabriel Costa Monteiro ; Silva-Vignato, Barbara ; Afonso, Juliana ; Freitas, Felipe Andre Oliveira ; Reecy, James Mark ; Koltes, James Eugene ; Koltes, Dawn ; Garrick, Dorian ; de Almeida Regitano, Luciana Correia ; de Carvalho Balieiro, Julio Cesar ; Mourao, Gerson Barreto ; Coutinho, Luiz Lehmann ; Fukumasu, Heidge ; Poleti, Mirele Daiana ; de Alencar, Severino Matias ; Luchiari Filho, Albino ; Cesar, Aline Silva Mello
Total Authors: 21
Document type: Journal article
Source: FUNCTIONAL & INTEGRATIVE GENOMICS; v. 23, n. 1, p. 19-pg., 2023-03-01.
Abstract

Pork is of great importance in world trade and represents the largest source of fatty acids in the human diet. Lipid sources such as soybean oil (SOY), canola (CO), and fish oil (FO) are used in pig diets and influence blood parameters and the ratio of deposited fatty acids. In this study, the main objective was to evaluate changes in gene expression in porcine skeletal muscle tissue resulting from the dietary oil sources and to identify metabolic pathways and biological process networks through RNA-Seq. The addition of FO in the diet of pigs led to intramuscular lipid with a higher FA profile composition of C20:5 n-3, C22:6 n-3, and SFA (C16:0 and C18:0). Blood parameters for the FO group showed lower cholesterol and HDL content compared with CO and SOY groups. Skeletal muscle transcriptome analyses revealed 65 differentially expressed genes (DEG, FDR 10%) between CO vs SOY, and 32 DEG for CO vs FO, and 531 DEG for SOY vs FO comparison. Several genes, including AZGP1, PDE3B, APOE, PLIN1, and LIPS, were found to be down-regulated in the diet of the SOY group compared to the FO group. The enrichment analysis revealed DEG involved in lipid metabolism, metabolic diseases, and inflammation between the oil groups, with specific gene functions in each group and altered blood parameters. The results provide mechanisms to help us understand the behavior of genes according to fatty acids. (AU)

FAPESP's process: 21/06553-8 - Association between biochemical parameters of the blood serum of pigs fed with different sources of fatty acids and the transcriptomic profile of the liver
Grantee:Julia Dezen Gomes
Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
FAPESP's process: 20/10042-6 - Lipid profile and transcriptome of the brain of swine feeded with different oil sources
Grantee:Bruna Pereira Martins da Silva
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/15653-3 - Effect of dietary oleic acid on fatty acid composition and inflammatory response profile in different tissues of pigs
Grantee:Aline Silva Mello Cesar
Support Opportunities: Scholarships in Brazil - Young Researchers
FAPESP's process: 18/26816-0 - Effect of dietary oleic acid on fatty acid composition and inflammatory response profile in different tissues of pigs
Grantee:Julia Dezen Gomes
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/02493-7 - Mammary tumors of dogs and the cancer stem cell theory: a comparative and translational approach
Grantee:Heidge Fukumasu
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/25180-2 - Effect of dietary oleic acid on fatty acid composition and inflammatory response profile in different tissues of pigs
Grantee:Aline Silva Mello Cesar
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/26797-6 - Effect of dietary oleic acid on fatty acid composition and inflammatory response profile in different tissues of pigs
Grantee:Julia Pereira Martins da Silva
Support Opportunities: Scholarships in Brazil - Scientific Initiation