In Vitro Schistosomicidal Activity of Some Brazilian Cerrado Species and Their Isolated Compounds

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Cunha, Nayanne Larissa ; de Mendonca Uchoa, Camila Jacintho ; Cintra, Lucas Silva ; de Souza, Herbert Cristian ; Peixoto, Juliana Andrade ; Silva, Claudia Peres ; Magalhaes, Lizandra Guidi ; Meleiro Gimenez, Valeria Maria ; Groppo, Milton ; Rodrigues, Vanderlei ; da Silva Filho, Ademar Alves ; Andrade e Silva, Marcio Luis ; Cunha, Wilson Roberto ; Pauletti, Patricia Mendonca ; Januario, Ana Helena

• Document type: Journal article
• Source: Evidence-based Complementary and Alternative Medicine; v. 2012, p. 8-pg., 2012-01-01.

Abstract

Miconia langsdorffii Cogn. (Melastomataceae), Roupala montana Aubl. (Proteaceae), Struthanthus syringifolius (Mart.) (Loranthaceae), and Schefflera vinosa (Cham. & Schltdl.) Frodin (Araliaceae) are plant species from the Brazilian Cerrado whose schistosomicidal potential has not yet been described. The crude extracts, fractions, the triterpenes betulin, oleanolic acid, ursolic acid and the flavonoids quercetin 3-O-beta-D-rhamnoside, quercetin 3-O-beta-D-glucoside, quercetin 3-O-beta-D-glucopyranosyl-(1-2)-alpha-L-rhamnopyranoside and isorhamnetin 3-O-beta-D-glucopyranosyl-(1-2)-alpha-L-rhamnopyranoside were evaluated in vitro against Schistosoma mansoni adult worms and the bioactive n-hexane fractions of the mentioned species were also analyzed by GC-MS. Betulin was able to cause worm death percentage values of 25% after 120 h (at 100 mu M), and 25% and 50% after 24 and 120 h (at 200 mu M), respectively; besides the flavonoid quercetin 3-O-beta-D-rhamnoside promoted 25% of death of the parasites at 100 mu M. Farther the flavonoids quercetin 3-O-beta-D-glucoside and quercetin 3-O-beta-D-rhamnoside at 100 mu M exhibited significantly reduction in motor activity, 75% and 87.5%, respectively. Biological results indicated that crude extracts of R. montana, S. vinosa, and M. langsdorffii and some n-hexane and EtOAc fractions of this species were able to induce worm death to some extent. The results suggest that lupane-type triterpenes and flavonoid monoglycosides should be considered for further antiparasites studies. (AU)

• FAPESP's process: 08/01268-9 - Phytochemical study and evaluation of the antimalarial, antimicrobial and acetylcholinesterase inhibition activities from the São Paulo State cerrado native species
• Grantee: Ana Helena Januário
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/00604-8 - Molecules of interest: isolation, identification,structural modifications and evaluation by several biological assays
• Grantee: Wilson Roberto Cunha
• Support Opportunities: Regular Research Grants