Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay

Author(s)

de Oliveira, Tassia Regina ; Martucci, Diego Henrique ; Faria, Ronaldo Censi

• Document type: Journal article
• Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 255, p. 8-pg., 2018-02-01.

Abstract

Herein, we describe the construction of a simple, low-cost, and fully disposable microfluidic device (D mu FD) for ultrasensitive Salmonella typhimurium (S.typhi) detection. The D mu FD is based on unmodified carbon electrode array for rapid detection of S. typhi in milk samples by magneto-immunoassay using gold nanoparticles (AuNPs) as a label. In the method proposed, S. typhi was magnetically captured and separated from samples using magnetic beads modified with a monoclonal anti-Salmonella antibody followed by the addition of polyclonal anti-Salmonella antibody labeling with AuNPs. The magneto-immunoconjugate formed was injected into the D mu FD and captured on the electrode surface by placing magnets behind the unmodified working electrodes. The S. typhi detection was carried out by the electrochemical response of the gold from AuNPs. A single D mu FD was used for rapid and full S. typhi analysis by measuring standards and samples simultaneously. A low limit of detection (LOD) of 7.7 cells mL(-1) for S. typhi in a linear range from 10.0 to 100.0 cells mL(-1) was obtained. The D FD proved to be fast, accurate, and inexpensive for S. typhi detection and has great potential to multiplex detection of different food contaminants. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 14/21115-3 - Characterization of immunosensors array for detecting pathogenic micro-organisms in food by using a microfluidic system
• Grantee: Diego Henrique Martucci
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/22401-0 - Development of disposable immunosensors for detection of Alzheimer's Disease biomarkers
• Grantee: Tássia Regina de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/19890-1 - Development of methods for diagnosis of diseases using magnetic particles and an array of imunossensors in a disposable microfluidic system
• Grantee: Ronaldo Censi Faria
• Support Opportunities: Regular Research Grants