The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers

Author(s)

Sato, Tabata P. ; Rodrigues, Bruno V. M. ; Mello, Daphne C. R. ; Munchow, Eliseu A. ; Ribeiro, Juliana S. ; Machado, Joao Paulo B. ; Vasconcellos, Luana M. R. ; Lobo, Anderson O. ; Bottino, Marco C. ; Borges, Alexandre L. S.

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 5, p. 9-pg., 2020-10-13.

Abstract

Objectives This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers. Materials and methods nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey's test (alpha= 0.05). Results ChHa fibers demonstrated a bead-like morphology, with thinner (331 +/- 110 nm) and smoother (Ra = 2.9 +/- 0.3 mu m) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except forP. intermedia), and higher antibiofilm activity againstP. gingivalisbiofilm. Conclusions The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers. (AU)

• FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
• Grantee: Fernanda Roberta Marciano
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
• Grantee: Anderson de Oliveira Lobo
• Support Opportunities: Research Grants - Young Investigators Grants