Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models

Author(s)

Giacone, Daniela, V ; Dartora, Vanessa F. M. C. ; de Matos, Jenyffer K. R. ; Passos, Julia S. ; Miranda, Daniel A. G. ; de Oliveira, Erica A. ; Silveira, Edilberto R. ; Costa-Lotufo, Leticia, V ; Maria-Engler, Silvya S. ; Lopes, Luciana B.

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 165, p. 11-pg., 2020-12-15.

Abstract

Due to the limited options for topical management of skin cancer, this study aimed at developing and evaluating nanoemulsions (NE) for topical delivery of the cytotoxic agent piplartine (piperlongumine). NEs were modified with chitosan or sodium alginate, and the effects on the physicochemical properties, piplartine delivery and formulation efficacy were evaluated. The nanoemulsion droplets displayed similar size (96-112 nm), but opposite charge; the polysaccharides improved piplartine penetration into and across the skin (1.3-1.9-fold) in a similar manner, increasing the ratio "drug in the skin/receptor phase" by 1.4-1.5-fold compared to the plain NE and highlighting their relevance for cutaneous localization. Oleic acid addition to the chitosan-containing NE further increased drug penetration (similar to 1.9-2.0-fold), as did increases in drug content from 0.5 to 1%. The cytotoxicity of piplartine was similar to 2.8-fold higher when the drug was incorporated in the chitosan-containing NE compared to its solution (IC50 = 14.6 mu M) against melanoma cells. The effects of this nanocarrier on 3D melanoma tissues were concentration-related; at 1%, piplartine elicited marked epidermis destruction. These results support the potential applicability of the chitosan-modified nanoemulsion containing piplartine as a new strategy for local management of skin cancer. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 18/13877-1 - Nanocarriers for localized treatment and chemoprevention of breast tumors
• Grantee: Luciana Biagini Lopes
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 18/18813-1 - Bioresponsive gels containing nanoparticles for exudate control and co-delivery of propranolol and adenosine for wound healing applications
• Grantee: Jenyffer Kelly Rocha de Matos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/07856-9 - Cationic nanoemulsions for piplartine cutaneous delivery and treatment of skin tumors
• Grantee: Daniela Veronesi Giacone
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/04174-4 - Development and evaluation of cationic bioadhesive nanocarriers as a platform to localize piplartine in the breast for tumor treatment
• Grantee: Vanessa Franco Carvalho Dartora
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/01208-8 - Bioadhesive nanostructured systems for intraductal administration and localized treatment of Breast Cancer
• Grantee: Julia Sapienza Passos
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/17177-6 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds
• Grantee: Leticia Veras Costa Lotufo
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 16/16554-3 - Emerging genes in melanoma progression and chemoresistance
• Grantee: Érica Aparecida de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral