Bioresponsive gels containing nanoparticles for exudate control and co-delivery of propranolol and adenosine for wound healing applications

• Grant number: 18/18813-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2018
• Effective date (End): November 30, 2019
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Luciana Biagini Lopes
• Grantee: Jenyffer Kelly Rocha de Matos
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/16617-7 - Nanostructured systems for topical delivery and co-localization of chemopreventive and chemoterapeutic agents in the skin and breast tissue, AP.JP

Abstract

Impaired wound healing represents a biomedical burden, and the need for dressings with improved performance, flexibility and swelling properties stimulates continuous research. In this study, we propose to develop microemulsions that swell and form nanostructured liquid crystalline gels upon uptake of excess wound exudate, while providing sustained and local delivery of adenosine and propranolol to aid wound healing. We also propose to evaluate whether drug coencapsulation in alginate nanoaparticles prior to incorporation in the microemulsions further prolong release. Microemulsions will be developed with monolein, phosphatidylcholine, glycerol, tricaprilin and water. The relationship between composition and type of liquid crystalline phase formed, swelling kinetics and in vitro release will be studied. An optimized formulation will be selected for incorporation of alginate nanoparticles, which will be produced by ionotropic gelation using CaCl2. After characterization of size, zeta potential and drug entrapment, they will beincorporated in the microemulsions, and their influence on swelling kinetics and in vitro release will be studied.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIACONE, V, DANIELA; DARTORA, VANESSA F. M. C.; DE MATOS, JENYFFER K. R.; PASSOS, JULIA S.; MIRANDA, DANIEL A. G.; DE OLIVEIRA, ERICA A.; SILVEIRA, EDILBERTO R.; COSTA-LOTUFO, V, LETICIA; MARIA-ENGLER, SILVYA S.; LOPES, LUCIANA B.. Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models. International Journal of Biological Macromolecules, v. 165, n. A, p. 1055-1065, DEC 15 2020. (17/07856-9, 15/17177-6, 16/16554-3, 17/04174-4, 20/01208-8, 18/18813-1, 18/13877-1)

SANTOS, ROGERIO A.; RAE, MARIANA; DARTORA, VANESSA F. M. C.; MATOS, JENYFFER K. R.; CAMARINI, ROSANA; LOPES, LUCIANA B.. Bioresponsive nanostructured systems for sustained naltrexone release and treatment of alcohol use disorder: Development and biological evaluation. International Journal of Pharmaceutics, v. 585, JUL 30 2020. (17/04174-4, 18/05038-0, 18/13877-1, 13/16617-7, 18/18813-1, 15/02397-0)

CARVALHO, VANESSA F. M.; SALATA, GIOVANNA C.; DE MATOS, JENYFFER K. R.; COSTA-FERNANDEZ, SANDRA; CHORILLI, MARLUS; STEINER, ALEXANDRE A.; DE ARAUJO, GABRIEL L. B.; SILVEIRA, EDILBERTO R.; COSTA-LOTUFO, V, LETICIA; LOPES, LUCIANA B.. Optimization of composition and obtainment parameters of biocompatible nanoemulsions intended for intraductal administration of piplartine (piperlongumine) and mammary tissue targeting. International Journal of Pharmaceutics, v. 567, AUG 15 2019. (17/23213-0, 13/16617-7, 17/04174-4, 18/03418-0, 18/18813-1, 18/13877-1)

GIACONE, DANIELA, V; DARTORA, VANESSA F. M. C.; DE MATOS, JENYFFER K. R.; PASSOS, JULIA S.; MIRANDA, DANIEL A. G.; DE OLIVEIRA, ERICA A.; SILVEIRA, EDILBERTO R.; COSTA-LOTUFO, LETICIA, V; MARIA-ENGLER, SILVYA S.; LOPES, LUCIANA B.. Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models. International Journal of Biological Macromolecules, v. 165, p. 11-pg., 2020-12-15. (18/13877-1, 18/18813-1, 17/07856-9, 17/04174-4, 20/01208-8, 15/17177-6, 16/16554-3)