Bioadhesive nanostructured systems for intraductal administration and localized treatment of Breast Cancer

• Grant number: 20/01208-8
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): March 01, 2020
• Effective date (End): April 30, 2025
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Luciana Biagini Lopes
• Grantee: Julia Sapienza Passos
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/13877-1 - Nanocarriers for localized treatment and chemoprevention of breast tumors, AP.JP2
• Associated scholarship(s): 21/12664-7 - Synthesis of thermoresponsive nanostructured lipid carrier core with poly(N-isopropylacrylamide) shell nanoparticle for drug encapsulation and intraductal delivery into the mammary tissue, BE.EP.DD

Abstract

Breast Tumors have a high incidence worldwide, but there are a lack of effective and well-accepted alternatives for localized treatment of noninvasive forms of the disease, such as the Ductal Carcinoma In Situ (DCIS). In this project, we propose the development of polyelectrolyte nanoparticles for intraductal administration of cytotoxic drugs, in order to optimize the drug localization in breast tissue. These complexes will be developed using chitosan and hyaluronic acid, and incorporated into Poloxamer 407, a thermosensitive polymer, in order to obtain in vivo gelling systems, aiming to prolong the residence time of nanoparticles in the mammary ducts. The affinity between composition and drug incorporation will be studied in order to evaluate how composition affects the properties of the system, by characterization by particle size, polydispersion index and zeta potential. A formulation will then be selected (optimized formulation) to evaluate the bioadhesive properties, irritation potential, drug release and time of residence in the breast tissue. The influence of nanoencapsulation on its efficacy and selectivity will be evaluated on 2D tumor cultures and on 3D multicellular spheroids, by studying formulation influence on viability and proliferation of tumor and non-transformed cells. With this study, we aim to contribute to the development of a more effective and safe platform for the treatment of DCIS. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIACONE, V, DANIELA; DARTORA, VANESSA F. M. C.; DE MATOS, JENYFFER K. R.; PASSOS, JULIA S.; MIRANDA, DANIEL A. G.; DE OLIVEIRA, ERICA A.; SILVEIRA, EDILBERTO R.; COSTA-LOTUFO, V, LETICIA; MARIA-ENGLER, SILVYA S.; LOPES, LUCIANA B.. Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models. International Journal of Biological Macromolecules, v. 165, n. A, p. 1055-1065, DEC 15 2020. (17/07856-9, 15/17177-6, 16/16554-3, 17/04174-4, 20/01208-8, 18/18813-1, 18/13877-1)

MOJEIKO, GABRIELA; PASSOS, JULIA SAPIENZA; APOLINARIO, ALEXSANDRA CONCEICAO; LOPES, LUCIANA BIAGINI. opical transdermal chemoprevention of breast cancer: where will nanomedical approaches deliver us. Nanomedicine, v. 16, n. 19, JUL 2021. (18/13877-1, 18/14375-0, 19/00196-9, 20/01208-8, 14/50928-2)

GIACONE, DANIELA, V; DARTORA, VANESSA F. M. C.; DE MATOS, JENYFFER K. R.; PASSOS, JULIA S.; MIRANDA, DANIEL A. G.; DE OLIVEIRA, ERICA A.; SILVEIRA, EDILBERTO R.; COSTA-LOTUFO, LETICIA, V; MARIA-ENGLER, SILVYA S.; LOPES, LUCIANA B.. Effect of nanoemulsion modification with chitosan and sodium alginate on the topical delivery and efficacy of the cytotoxic agent piplartine in 2D and 3D skin cancer models. International Journal of Biological Macromolecules, v. 165, p. 11-pg., 2020-12-15. (18/13877-1, 18/18813-1, 17/07856-9, 17/04174-4, 20/01208-8, 15/17177-6, 16/16554-3)

DARTORA, VANESSA F. C.; SALATA, GIOVANNA C.; PASSOS, JULIA S.; BRANCO, PAOLA C.; SILVEIRA, EDILBERTO; STEINER, ALEXANDRE A.; V. COSTA-LOTUFO, LETICIA; LOPES, LUCIANA B.. Hyaluronic acid nanoemulsions improve piplartine cytotoxicity in 2D and 3D breast cancer models and reduce tumor development after intraductal administration. International Journal of Biological Macromolecules, v. 219, p. 12-pg., 2022-08-05. (14/50928-2, 18/17679-0, 18/03418-0, 20/01208-8, 17/04174-4, 15/17177-6)

PASSOS, JULIA SAPIENZA; DARTORA, VANESSA F. M. C.; SALATA, GIOVANNA CASSONE; MALAGO, ISABELLA DRASZESSKI; LOPES, LUCIANA B.. Contributions of nanotechnology to the intraductal drug delivery for local treatment and prevention of breast cancer. International Journal of Pharmaceutics, v. 635, p. 15-pg., 2023-02-14. (18/13877-1, 17/04174-4, 20/01208-8, 14/50928-2, 19/26048-6)