Diving into the proteomic atlas of SARS-CoV-2 infected cells

Author(s):Show less -

Carregari, Victor C. ; Reis-de-Oliveira, Guilherme ; Crunfli, Fernanda ; Smith, Bradley J. ; de Souza, Gabriela Fabiano ; Muraro, Stefanie Primon ; Saia-Cereda, Veronica M. ; Vendramini, Pedro H. ; Baldasso, Paulo A. ; Silva-Costa, Licia C. ; Zuccoli, Giuliana S. ; Brandao-Teles, Caroline ; Antunes, Andre ; Valenca, Aline F. ; Davanzo, Gustavo G. ; Virgillio-da-Silva, Joao Victor ; Araujo, Thiago dos Reis ; Guimaraes, Raphael Campos ; Chaim, Felipe David Mendonca ; Chaim, Elinton Adami ; Onodera, Carolina Mie Kawagosi ; Ludwig, Raissa Guimaraes ; Saccon, Tatiana Dandolini ; Damasio, Andre R. L. ; Leiria, Luiz Osorio S. ; Vinolo, Marco Aurelio R. ; Farias, Alessandro S. ; Moraes-Vieira, Pedro M. ; Mori, Marcelo A. ; Modena, Jose Luiz P. ; Martins-de-Souza, Daniel

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 14, n. 1, p. 14-pg., 2024-03-28.

Abstract

The COVID-19 pandemic was initiated by the rapid spread of a SARS-CoV-2 strain. Though mainly classified as a respiratory disease, SARS-CoV-2 infects multiple tissues throughout the human body, leading to a wide range of symptoms in patients. To better understand how SARS-CoV-2 affects the proteome from cells with different ontologies, this work generated an infectome atlas of 9 cell models, including cells from brain, blood, digestive system, and adipocyte tissue. Our data shows that SARS-CoV-2 infection mainly trigger dysregulations on proteins related to cellular structure and energy metabolism. Despite these pivotal processes, heterogeneity of infection was also observed, highlighting many proteins and pathways uniquely dysregulated in one cell type or ontological group. These data have been made searchable online via a tool that will permit future submissions of proteomic data (https://reisdeoliveira.shinyapps.io/Infectome_App/) to enrich and expand this knowledgebase. (AU)

• FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
• Grantee: Licio Augusto Velloso
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/07559-3 - BRAINN - The Brazilian Institute of Neuroscience and Neurotechnology
• Grantee: Fernando Cendes
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 20/05601-6 - Neutrophil extracelular traps (NETs): role in the pathophysiology and potential as a therapeutic target on COVID-19
• Grantee: Fernando de Queiroz Cunha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/10068-4 - Multi-User Equipment approved in grant 13/08711-3: mass spectrometer waters SYNAPT G2-Si HDMS + nanoACQUITY UPLC
• Grantee: Daniel Martins-de-Souza
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 17/25588-1 - From the basic understanding to clinical biomarkers to schizophrenia: a neuroproteomics-centered multidisciplinary study
• Grantee: Daniel Martins-de-Souza
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
• Grantee: Fernando de Queiroz Cunha
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 19/11457-8 - Multifactorial analysis of anxiety disorders and depression in patients with epilepsy and caregivers
• Grantee: Rafael Batista João
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/00098-7 - Multi-User Equipment approved in grant 2017/25588-1: cromatógrafo Acquity UPLC I-Class
• Grantee: Daniel Martins-de-Souza
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 20/04919-2 - Determining molecular candidates that contribute to the higher risk of COVID-19 in aged individuals
• Grantee: Marcelo Alves da Silva Mori
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/04860-8 - Global genome screening with CRISPRko libraries to identify essential factors in SARS-CoV2 infection and replication
• Grantee: Thiago Mattar Cunha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/04746-0 - Understanding the molecular bases and the role of risk factors associated to SARS-CoV-2 infection in preclinical models
• Grantee: Daniel Martins-de-Souza
• Support Opportunities: Regular Research Grants