Disposable and eco-friendly electrochemical immunosensor for rapid detection of SARS-CoV-2

Author(s):Show less -

Rocha, Danielly S. ; Baldo, Thaisa A. ; Silva-Neto, Habdias A. ; Duarte-Junior, Gerson F. ; Bazilio, Gabriela S. ; Borges, Clayton L. ; Parente-Rocha, Juliana A. ; de Araujo, William R. ; de Siervo, Abner ; Paixa, Thiago L. R. C. ; Coltro, Wendell K. T.

• Document type: Journal article
• Source: Talanta; v. 268, p. 9-pg., 2023-10-29.

Abstract

This study describes the development of a simple, disposable, and eco-friendly electrochemical immunosensor for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Electrochemical devices were manufactured by stencil-printing using low-cost materials such as polyester sheets, graphite flakes, and natural resin. The immunosensor comprises gold nanoparticles stabilized with cysteamine, glutaraldehyde, anti-SARS-CoV-2 S protein monoclonal antibody (Ab1) as the biological receptor, and bovine serum albumin as a protec-tive layer. The COVID-19 diagnostic was based on rapid square wave voltammetry measurements (15 min) using [Fe(CN)6]3-/4-as a redox probe. The method presented a linear response in the concentration range from 250 pg mL-1 to 20 mu g mL-1 S protein, with a limit of detection of 36.3 pg mL-1. The proposed immunosensor was stable for up to two weeks when stored at 4 degrees C and it demonstrated excellent clinical performance in diagnosing COVID-19 when applied to a panel of 44 undiluted swab samples collected from symptomatic patients. In comparison with results obtained through the quantitative reverse transcription polymerase chain reaction method, the proposed immunosensor offered 100 % accuracy, thus emerging as a powerful alternative candidate for routine and decentralized testing, which can be helpful in controlling the COVID-19 outbreak. (AU)

• FAPESP's process: 07/54829-5 - Electronic and geometric structure of nano-materials: synchrotron radiation studies
• Grantee: Richard Landers
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 07/08244-5 - Study of the magnetic properties of Pd and PdAu ultrathin films and nanoparticles by using XMCD
• Grantee: Abner de Siervo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/03250-7 - Development of portable and cost-effective biosensors for clinical diagnosis of bacterial and viral infections
• Grantee: William Reis de Araujo
• Support Opportunities: Research Grants - Initial Project