In Vitro Evaluation of Bacterial Leakage Along the Implant-Abutment Interface of an External-Hex Implant After Saliva Incubation

Author(s)

do Nascimento, Cassio ^[1] ; Miani, Paola Kirsten ^{[1, 2]} ; Watanabe, Evandro ; Pedrazzi, Vinicius ^[1] ; de Albuquerque, Jr., Rubens Ferreira ^{[1, 3]}

Affiliation

^[1] Univ Sao Paulo, Fac Dent Ribeirao Preto, Dept Dent Mat & Prosthodont, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Toxicol & Bromatol Clin Anal, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] McGill Univ, Fac Dent, Montreal, PQ H3A 2T5 - Canada

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS; v. 26, n. 4, p. 782-787, JUL-AUG 2011.
• Web of Science Citations: 21

Abstract

The aim of this in vitro study was to evaluate bacterial leakage along the implant-abutment interface under unloaded conditions. Twelve premachined abutments with plastic sleeves and 12 dental implants were used in this study. Prior to tests of bacterial leakage, samples from the inner parts of the implants were collected with sterile microbrushes to serve as negative controls for contamination. After casting, the abutments were tightened to 32 Ncm on the implants. The assemblies were immersed in 2.0 mL of human saliva and incubated for 7 days. After this period, possible contamination of the internal parts of the implants was evaluated using the DNA Checkerboard method. Microorganisms were found in the internal surfaces of all the implants evaluated. Aggregatibacter actinomycetemcomitans and Capnocytophaga gingivalis were the most incident species. No microorganisms were found in the samples recovered from the implants before contamination testing (negative control). Bacterial species from human saliva may penetrate the implant-abutment interface under unloaded conditions. INT J ORAL MAXILLOFAC IMPLANTS 2011;26:782-787 (AU)