Employment of Magnetic Poly(Styrene-co-Triethylene Gycol Dimethacrylate) as an Immobilization Matrix for Lipase G: Application of Hexyl Oleate Synthesis and Kinetic Study

Author(s)

de Assis, Gabrielle P. ; Silva, Vinicius S. ; da Silva, Mateus V. C. ; Rangel, Amanda B. S. ; Aguiar, Leandro G. ; de Freitas, Larissa

• Document type: Journal article
• Source: CATALYSIS LETTERS; v. N/A, p. 11-pg., 2024-10-07.

Abstract

This study aimed to synthesize the emollient ester hexyl oleate by enzymatic catalysis using lipase G from Penicillium camemberti immobilized on the magnetic copolymer poly(styrene-co-triethylene glycol dimethacrylate) (STY-TEGDMA-M). For this, an experimental study was carried out on esterification reactions conducted in solvent-free medium, and the collected data were used to construct a mathematical model. The physical properties of the immobilized derivative were evaluated by scanning electron microscopy and Fourier-transform infrared spectroscopy. The results confirmed that lipase G was successfully immobilized onto the support, with a catalytic activity of 564.63 U g-1. The biocatalyst showed optimum performance at 45 degrees C, reaching 82% conversion after 48 h of reaction. A chromatographic method was used to confirm the formation of hexyl oleate. A thermal stability assay was conducted, showing that the biocatalyst retained 87% of its initial activity after 4 h of incubation at 60 degrees C. Mathematical modeling was based on the ping-pong bi-bi kinetic mechanism. The best model was selected according to the lowest value of the corrected Akaike information criterion (AICC = 124.776). The model revealed equilibrium constant values (Keq) ranging from 0.87 to 14.57, maximum rates of the forward reaction ranging from 0.088 to 0.090 mol L-1 min-1, and a maximum rate of the reverse reaction of 0.021 mol L-1 min-1. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 16/17833-3 - Emollient esters synthesis catalyzed by immobilized lipase on styrene-based polymers with magnetic properties
• Grantee: Larissa de Freitas
• Support Opportunities: Regular Research Grants
• FAPESP's process: 23/04243-7 - "Application of green technology for remediation of environmental pollution using heterogeneous catalysts prepared with alternative supports"
• Grantee: Larissa de Freitas
• Support Opportunities: Regular Research Grants