Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis

Author(s)

Michaliski, Lamonielli F. ; Ioca, Laura P. ; Oliveira, Leandro S. ; Crnkovic, Camila M. ; Takaki, Mirelle ; Freire, Vitor F. ; Berlinck, Roberto G. S.

• Document type: Journal article
• Source: METHODS AND PROTOCOLS; v. 6, n. 5, p. 18-pg., 2023-10-01.

Abstract

Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products. The overall procedure typically requires 3-4 months of work when first developed, and up to 3 months as a routine procedure. (AU)

• FAPESP's process: 19/07894-3 - Discovery of bioactive secondary metabolites produced by microbial strains from isolated environments and study of the biosynthesis of alpha-aminopyrones produced by Aspergillus sp. DLM38
• Grantee: Lamonielli Fagá Michaliski
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/01017-0 - Multi-use instrument approved and awarded in the project 2013/50228-8, name of instrument: ultra-performance liquid chromatograph coupled with a high resolution mass spectrometer
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 22/07831-4 - Biosynthesis of phomactins produced by the fungus Biatriospora sp. CBMAI 1333 and alpha-aminopyrones produced by the fungus Aspergillus sp. DLM38
• Grantee: Lamonielli Fagá Michaliski
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• FAPESP's process: 16/05133-7 - Study of marine-derived microrganisms metabolism: chemical profile and biosynthesis
• Grantee: Laura Pavan Ióca
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/14114-3 - Which metabolomics components influence the phytopathogenicity of Colletotrichum spp.?
• Grantee: Mirelle Takaki
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/17721-9 - The role of Chemistry in holobiont adaptation
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/10742-8 - The role of a nonribosomal peptide in promoting swarming in Pseudovibrio brasiliensis Ab134 and related bacteria
• Grantee: Laura Pavan Ióca
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/03679-8 - Use of statistical tools and molecular networking for the metabolomics analysis of Colletotrichum spp
• Grantee: Mirelle Takaki
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 19/00447-1 - Improving and diversifying the production and investigating the biosynthesis of phomactins by the cultured fungus Biatriospora sp. CBMAI 1333
• Grantee: Camila Manoel Crnkovic
• Support Opportunities: Scholarships in Brazil - Post-Doctoral