| Full text | |
| Author(s): |
Grajales, Lina Maria
;
Wang, Hailei
;
Casciatori, Fernanda Perpetua
;
Thomeo, Joao Claudio
Total Authors: 4
|
| Document type: | Journal article |
| Source: | CHEMICAL ENGINEERING AND PROCESSING; v. 210, p. 11-pg., 2025-04-01. |
| Abstract | |
Cellulolytic enzymes are vital for converting cellulosic residues into biofuels, yet large-scale production through solid-state cultivation (SSC) remains challenging due to the lack of suitable bioreactors. This study addresses this issue by developing a rotary drum bioreactor to produce cellulases from the thermophilic fungus Myceliophthora thermophila I-1D3b, using sugarcane bagasse and wheat bran as substrates. The bioreactor integrates upstream, fermentation, and downstream processes, streamlining production and enhancing efficiency. The study explored enzymatic activity (EA) at varying substrate loadings and drum rotation conditions. Although statistically similar, at 50 % loading, drum rotation slightly improved EA (49.12 U/mL f 6.56 U/mL) compared to static conditions (47.78 U/mL f 8.25 U/mL). Conversely, at 40 % loading, rotation reduced EA significantly (23.57 U/ mL f 3.17 U/mL) compared to static conditions (46.91 U/mL f 8.17 U/mL). At 60 % loading, EA was similar under both static and rotated conditions. The design effectively supports fermentation, facilitates enzymatic extract recovery, and minimizes temperature and moisture gradients. These results demonstrate the rotary drum bioreactor's potential for scaling up cellulase production, offering a promising solution for industrial SSC processes. (AU) | |
| FAPESP's process: | 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales |
| Grantee: | Roberto de Campos Giordano |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 18/00996-2 - Development of a pseudo-continuous packed-bed bioreactor for solid-state fermentation |
| Grantee: | Fernanda Perpétua Casciatori |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 14/23453-3 - Packed-bed solid-state fermentation bioreactor scale-up for cellulase production |
| Grantee: | João Cláudio Thoméo |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |