Impact of hydroethanolic extracts from Guaco leaves (Mikania glomerata Sprengel) on mechanical properties and bioactivities of gelatin-based bioactive films

Author(s)

Bertan, David Willian ; Lima, Luciana Ribeiro da Silva ; Benoso, Paula ; Lourenco, Rodrigo Vinicius ; Bittante, Ana Monica Quinta Barbosa ; Gomes, Andresa ; Moraes, Izabel Cristina Freitas ; Ferreira, Mariana Simoes Larraz ; Sobral, Paulo Jose do Amaral

• Document type: Journal article
• Source: FOOD AND BIOPRODUCTS PROCESSING; v. 151, p. 17-pg., 2025-04-26.

Abstract

Guaco leaves (Mikania glomerata Sprengel) show potential as a source of phenolic compounds (PC) that can be extracted for application in active film production. The phenolic profile of guaco leaf hydroethanolic extracts (GLHE) and their impact on the properties of the gelatin-glycerol film-forming solutions (FFS) and films were evaluated. GLHE were prepared using 20 (GLHE.20), 40 (GLHE.40), and 60 % (v/v) ethanol (GLHE.60), and incorporated into gelatin FFS at concentrations of 50 or 150 (g/100 g gelatin). The phenolic profile of GLHE was determined by untargeted metabolomics. The FFS and their films were characterized. Phenolic acids and flavonoids comprised 75 % of the PC and significantly influenced the phenolic profile of the extracts. Multivariate analyses suggested that derivatives of caffeic and coumaric acids, along with various flavonoids, contributed to the strong antioxidant activity of GLHE.40 and GLHE.60. The addition of these extracts into films did not affect their microstructure, crystallinity, thickness, moisture content, solubility in water, and opacity. On the other hand, thermal properties, water contact angle, water vapor permeability, mechanical properties, and color parameters. Moreover, the addition of GLHE increased the antioxidant activity (AoA) of the material, without, however, conferring antimicrobial activity against Gram-positive or Gram-negative bacteria. A good stability of the AoA within the gelatin matrix was observed over a six-month period. Overall, the type of solvent affected the films' tensile strength (TS) (GLHE.20 (AU)

• FAPESP's process: 19/26348-0 - Encapsulation of non-polar bioactive agents in conventional emulsions, Pickering Emulsions, or nanoemulsions: application in active films, and activity conservation and controlled release studies
• Grantee: Andresa Gomes Brunassi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC