Periodontal Tissue Engineering After Tooth Replantation

Author(s)

da Silva Assuncao, Luciana Reichert ^[1] ; Colenci, Renato ^[2] ; Ferreira do-Amaral, Caril Constante ^[3] ; Sonoda, Celso Koogi ^[4] ; Mogami Bomfim, Suely Regina ^[5] ; Okamoto, Roberta ^[4] ; Golim, Marjorie de Assis ^[6] ; Deffune, Elenice ^[2] ; Percinoto, Celio ^[7] ; Penha de Oliveira, Sandra Helena ^[3]

Affiliation

^[1] Fed Univ Para UFPA, Sch Dent, BR-66075900 Belem, Para - Brazil

^[2] UNESP Univ Estadual Paulista, Fac Med, Lab Cellular Engn, Botucatu Blood Ctr, Sao Paulo - Brazil

^[3] UNESP Univ Estadual Paulista, Sch Dent, Dept Basic Sci, Sao Paulo - Brazil

^[4] UNESP Univ Estadual Paulista, Sch Dent, Dept Surg & Integrated Clin, Sao Paulo - Brazil

^[5] UNESP Univ Estadual Paulista, Sch Dent, Dept Clin & Surg & Anim Reprod, Vet Med Course, Sao Paulo - Brazil

^[6] UNESP Univ Estadual Paulista, Fac Med, Botucatu Blood Ctr, Lab Flow Cytometry, Sao Paulo - Brazil

^[7] UNESP Univ Estadual Paulista, Sch Dent, Dept Pediat Dent, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Periodontology; v. 82, n. 5, p. 758-766, MAY 2011.
• Web of Science Citations: 12

Abstract

Background: Blood-derived products, platelet-poor plasma (PPP) and platelet-rich plasma (PRP), constitute an approach in the enhancement of tissue healing. PRP has also been used as a scaffold for bone marrow stem cells in tissue engineering. This study evaluates the effect of PPP, calcium chloride activated PRP (PRP/Ca), calcium chloride and thrombin-activated PRP (PRP/Thr/Ca), and bone marrow mononuclear cells and PRP/Ca (BMMCs/PRP/Ca) on the healing of replanted dog teeth. Methods: After 30 minutes of extraction, teeth were replanted with 1) no material (control); 2) PPP; 3) PRP/Ca; 4) PRP/Thr/Ca; or 5) BMMCs/PRP/Ca. Histologic, histomorphometric, and immunohistochemical analysis was assessed 120 days after replantation. Data from histomorphometric analysis were analyzed statistically (analysis of variance, Tukey; P < 0.05). Quantitative immunohistochemical analysis was analyzed by Kruskal-Wallis and Dunn post hoc test (P < 0.05). Results: Flow cytometry analysis showed 55.98% of CD34(+) and 32.67% of CD90/Thy-1 for BMMCs sample. BMMCs/PRP/Ca presented the largest areas of replacement resorption characterized by osseous ingrowth into cementum (P < 0.05), with intense immunomarcation for tartrate-resistant acid phosphatase. The PRP/Ca group also showed areas of replacement resorption with significant immunomarcation for osteopontin. PRP/Thr/Ca presented no replacement resorption. PPP showed areas of inflammatory resorption, with immunomarcation for tartrate-resistant acid phosphatase. Conclusions: The results suggest that platelets activated with thrombin play an important role in the healing of tissues after tooth replantation. Additional studies are necessary to test other materials, because PRP/Ca did not present an appropriate scaffold for undifferentiated cells in the treatment of avulsed teeth. J Periodontol 2011;82:758-766. (AU)