| Full text | |
| Author(s): Show less - |
Bertonha, Ariane F.
;
Williams, David E.
;
Nicacio, Karen J.
;
de Amorim, Marcelo R.
;
Schoellhorn, Sydney M.
;
Aguiar, Anna Caroline C.
;
Valdes, Talita Alvarenga
;
Mendes, Giovana Rossi
;
Moura, Igor M. R.
;
Michaliski, Lamonielli F.
;
Gotha, Matheus
;
Zuccarino, Caue A. W.
;
Sette, Lara D.
;
Ferreira, Antonio G.
;
Andersen, Raymond J.
;
Guido, Rafael Victorio Carvalho
;
Berlinck, Roberto G. S.
Total Authors: 17
|
| Document type: | Journal article |
| Source: | ACS OMEGA; v. 10, n. 41, p. 15-pg., 2025-10-10. |
| Abstract | |
Peptaibols are modified linear peptides that typically include an acyl fragment connected at the N-terminal moiety, a reduced acid extremity and alpha-aminoisobutyric acid residues as common features, as well as other structural modifications. Peptaibols very often display potent biological activity, in particular, antimicrobial activity. Trichokonins VI (1) and VIII (2) are large peptaibols composed of 20 amino acids, which were previously only characterized by analysis of MS/MS data. We herein report the first full characterization of trichokonins VI (1) and VIII (2) by analysis of NMR data, along with an analysis by electronic circular dichroism and HRMS/MS data. Also, full genome sequencing and analysis of Trichoderma sp. L2-2 allowed us to identify a peptaibol biosynthesis gene cluster and the proposal for a biosynthetic assembly of trichokonins VI and VIII. Trichokonins VI and VIII also demonstrated antiplasmodial activity at the submicromolar range against Plasmodium falciparum. (AU) | |
| FAPESP's process: | 23/17676-9 - Biodiscovery and biosynthesis of secondary metabolites from bacteria and marine fungi |
| Grantee: | Matheus Gotha |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery |
| Grantee: | Glaucius Oliva |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 21/03977-1 - Discovery of inhibitors from Brazilian Savana plants as lead compound candidates for Malaria |
| Grantee: | Igor Mota Rodrigues de Moura |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 21/07343-7 - Isolation and identification of hidrossoluble bioactive peptaibols from produced by the fungus Hypodrea sp. |
| Grantee: | Matheus Gotha |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 20/01229-5 - Bioactive metabolites of bacterial flora microorganisms from non-filtering marine animals |
| Grantee: | Marcelo Rodrigues de Amorim |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 15/18192-9 - Discovery and development of Antimalarial Drugs: Structural Biology, Medicinal Chemistry and Parasitology |
| Grantee: | Anna Caroline Campos Aguiar |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 24/04949-0 - Discovery of natural products isolated from fungal lineages as lead candidates for Malaria |
| Grantee: | Talita Alvarenga Valdes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 15/01017-0 - Multi-use instrument approved and awarded in the project 2013/50228-8, name of instrument: ultra-performance liquid chromatograph coupled with a high resolution mass spectrometer |
| Grantee: | Roberto Gomes de Souza Berlinck |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 24/04805-8 - Integrated investigation of inhibitors active on infectious diseases: insights into molecular and cellular mechanisms of function |
| Grantee: | Rafael Victorio Carvalho Guido |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 22/07831-4 - Biosynthesis of phomactins produced by the fungus Biatriospora sp. CBMAI 1333 and alpha-aminopyrones produced by the fungus Aspergillus sp. DLM38 |
| Grantee: | Lamonielli Fagá Michaliski |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate (Direct) |
| FAPESP's process: | 22/01063-5 - Characterization of the antiplasmodial activity of natural products isolated from fungal metabolites as lead candidates for Malaria |
| Grantee: | Giovana Rossi Mendes |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 18/10742-8 - The role of a nonribosomal peptide in promoting swarming in Pseudovibrio brasiliensis Ab134 and related bacteria |
| Grantee: | Laura Pavan Ióca |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 19/07894-3 - Discovery of bioactive secondary metabolites produced by microbial strains from isolated environments and study of the biosynthesis of alpha-aminopyrones produced by Aspergillus sp. DLM38 |
| Grantee: | Lamonielli Fagá Michaliski |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 19/17721-9 - The role of Chemistry in holobiont adaptation |
| Grantee: | Roberto Gomes de Souza Berlinck |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/05133-7 - Study of marine-derived microrganisms metabolism: chemical profile and biosynthesis |
| Grantee: | Laura Pavan Ióca |
| Support Opportunities: | Scholarships in Brazil - Doctorate |